Human renal‐cell carcinoma tissue contains dendritic cells
Human renal‐cell carcinoma tissue contains dendritic cells
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DOI：
10.1002/(sici)1097-0215(19960927)68:1
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发表时间：
1996-09
影响因子：
6.4
通讯作者：
M. Thurnher;C. Radmayr;R. Ramoner;S. Ebner;G. Böck;H. Klocker;N. Romani;G. Bartsch
中科院分区：
文献类型：
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作者：
M. Thurnher;C. Radmayr;R. Ramoner;S. Ebner;G. Böck;H. Klocker;N. Romani;G. Bartsch
癌症的免疫监视需要抗原呈递细胞，其激活对肿瘤相关抗原特异性的T细胞。我们在这里表明，大量的树突状细胞，这是最有效的抗原呈递细胞，从器官培养中的肾肿瘤外植体迁移。肿瘤来源的树突状细胞具有成熟树突状细胞的所有特征。树突状细胞可以通过典型的胞质突起（=面纱）来识别。它们表达高水平的MHC产物和共刺激分子CD 86（B7 - 2）。树突状细胞表达CD 45 RO亚型，但不表达CD 45 RA。最重要的一点是，高达9%的迁移白细胞表达CD 83抗原，这是成熟树突状细胞的特异性标志物。与外周血相比，CD 83+细胞在肿瘤组织中富集约40倍。与培养的血液树突状细胞相比，肿瘤迁移树突状细胞捕获可溶性抗原的潜力降低，如荧光葡聚糖分子的排除所示。最后，在混合白细胞反应中，肿瘤来源的树突状细胞能够刺激来自脐带血的幼稚T细胞，这是树突状细胞的独特特征。这项研究表明，真正的树突状细胞存在于或浸润肾细胞癌组织中。尽管肿瘤组织中存在专职抗原呈递细胞，但肾细胞癌患者未能产生抗肿瘤免疫应答，这表明肿瘤相关树突状细胞以与肿瘤浸润淋巴细胞相似的方式被原位抑制。© 1996 Wiley利斯公司
Immune surveillance of cancer requires antigen‐presenting cells which activate T cells specific for tumor‐associated antigens. We show here that substantial numbers of dendritic cells, which are the most potent antigen‐presenting cells, emigrate from renal‐tumor explants in organ culture. Tumor‐derived dendritic cells presented with all characteristics of mature dendritic cells. Dendritic cells could be identified by typical cytoplasmic projections (=veils). They expressed high levels of MHC products and of the co‐stimulator CD86 (B7‐2). Dendritic cells expressed the CD45RO isoform but not CD45RA. The most important point was that up to 9% of the emigrating leukocytes expressed the CD83 antigen, a specific marker for mature dendritic cells. CD83+ cells were approximately 40‐fold enriched in the tumor tissue as compared to the peripheral blood. In contrast to cultured blood dendritic cells, tumor‐emigrant dendritic cells had a reduced potential to capture soluble antigen, as shown by the exclusion of fluoresceinated Dextran molecules. Finally, in mixed leukocyte reactions, tumor‐derived dendritic cells were able to stimulate naive T cells from cord blood, which is a unique feature of dendritic cells. This study demonstrates that genuine dendritic cells reside in or infiltrate renal‐cell carcinoma tissue. The failure of patients with renal‐cell carcinoma to mount an anti‐tumor immune response despite the presence of professional antigen‐presenting cells in the tumor tissue suggests that tumor‐associated dendritic cells are suppressed in situ, in a similar way to that described for tumor‐infiltrating lymphocytes. © 1996 Wiley‐Liss, Inc.