Detection and characterization of SNPs useful for identity control and parentage testing in major European dairy breeds
Detection and characterization of SNPs useful for identity control and parentage testing in major European dairy breeds
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DOI：
10.1046/j.1365-2052.2003.01071.x
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发表时间：
2004-02-01
期刊：
影响因子：
2.4
通讯作者：
Fries, R
中科院分区：
文献类型：
--
作者：
Werner, FAO;Durstewitz, G;Fries, R
我们建议使用单核苷酸多态性（SNP）代替多态性微卫星标记来进行牛的个体识别和亲子控制。为此，我们提出了一组初始的 37 个 SNP 标记以及性别特异性 SNP，用于荷斯坦、Fleckvieh 和 Braunvieh 品种的身份控制和亲子关系测试。为了获得合适的 SNP，总共筛选了 91.13 kb 的随机基因组 DNA，产生了 531 个 SNP。这些以及之前确定的 43 个 SNP 均遵循以下选择标准：(1) 在三个检查品种中的至少两个中，次要等位基因的频率必须大于 0.1，(2) 标记不应紧密连锁。通过分析合并 DNA 的测序痕迹或对单个 DNA 样本进行基因分型来估计等位基因频率。通过辐射杂交作图或荧光原位杂交对选定的 SNP 位点进行物理作图，并针对中性突变假设进行测试。理论上，所提出的标记集允许个体验证的身份概率小于 10(-13)，而亲子鉴定的排除能力超过 99.99%。
We propose the use of single nucleotide polymorphisms (SNPs) instead of polymorphic microsatellite markers for individual identification and parentage control in cattle. To this end, we present an initial set of 37 SNP markers together with a gender-specific SNP for identity control and parentage testing in the Holstein, Fleckvieh and Braunvieh breeds. To obtain suitable SNPs, a total of 91.13 kb of random genomic DNA was screened yielding 531 SNPs. These, and 43 previously identified SNPs, were subjected to the following selection criteria: (1) the frequency of the minor allele must be larger than 0.1 in at least two of the three examined breeds, and (2) markers should not be linked closely. Allele frequencies were estimated by analysing sequencing traces of pooled DNA or by genotyping individual DNA samples. The selected SNP loci were physically mapped by radiation hybrid mapping or by fluorescence in situ hybridization, and tested against the neutral mutation hypothesis. The presented marker set theoretically allows probabilities of identity less than 10(-13) for individual verification and exclusion powers exceeding 99.99% for parentage testing.