Chronic alcohol exposure induced gene expression changes in the zebrafish brain
Chronic alcohol exposure induced gene expression changes in the zebrafish brain
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DOI：
10.1016/j.bbr.2010.07.017
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发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Gerlai, Robert
中科院分区：
文献类型：
--
作者：
Pan, Yi;Kaiguo, Mo;Gerlai, Robert
慢性酒精暴露会影响中枢神经系统，影响行为，并诱导脊椎动物物种包括我们自己的神经适应性变化。由于酒精作用的复杂性，导致慢性酒精效应的分子机制尚未完全阐明。在这里，我们使用斑马鱼（酒精研究中的一种新型工具）来揭示大量对慢性酒精治疗有反应的基因。我们使用全基因组DNA微阵列证明了慢性酒精治疗的差异基因表达，并发现总共1914个基因显示出至少2倍的显著表达水平变化（1127个上调，787个下调）。这些基因中大约有三分之二以前没有已知的功能注释。微阵列分析的结果与通过定量实时RT-PCR分析的选定基因子集上获得的结果相关性良好。对具有已知注释的差异表达基因的分析富集了多种分子功能。这些已知基因中只有一小部分在文献中被报道与酒精有关。我们的结论是，斑马鱼是一个很好的工具，用于分析与酒精在脊椎动物中的作用相关的基因，这可能有助于发现和更好地理解酒精滥用的机制。(C)2010爱思唯尔有限公司版权所有。
Chronic alcohol exposure affects the central nervous system, influences behavior, and induces neuroadaptive changes in vertebrate species including our own. The molecular mechanisms responsible for chronic alcohol effects have not been fully elucidated due to the complexity of alcohol's actions. Here we use zebrafish, a novel tool in alcohol research, to reveal a large number of genes that respond to chronic alcohol treatment. We demonstrate differential gene expression in response to chronic alcohol treatment using full genome DNA microarrays and find a total of 1914 genes to show a minimum of 2-fold and significant expression level change (1127 were up- and 787 were down-regulated). Approximately two-thirds of these genes had no known previous functional annotation. The results of the microarray analyses correlated well with those obtained on a selected subset of genes analyzed by quantitative real-time RT-PCR. Analyses of the differentially expressed genes with known annotations were enriched for a variety of molecular functions. Only a fraction of these known genes has been reported in the literature to be alcohol related. We conclude that the zebrafish is an excellent tool for the analysis of genes associated with alcohol's actions in vertebrates, one which may facilitate the discovery and better understanding of the mechanisms of alcohol abuse. (C) 2010 Elsevier B.V. All rights reserved.