Disease duration determines canine distemper virus neurovirulence
Disease duration determines canine distemper virus neurovirulence
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DOI：
10.1128/jvi.00818-07
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发表时间：
2007-11-01
影响因子：
5.4
通讯作者：
von Messling, Veronika
中科院分区：
文献类型：
--
作者：
Bonami, Franqois;Rudd, Penny A.;von Messling, Veronika
麻疹病毒血凝素（H）蛋白介导与靶细胞的附着。为了评估其对犬瘟热病毒神经毒力的贡献，我们交换了野生型毒株5804 P和A75的H蛋白，并评估了嵌合病毒在雪貂中的发病机制。这两种菌株对雪貂都是致命的;然而，5804 P导致2周的疾病而没有神经系统体征，而A75与更长的病程和神经系统受累有关。我们观察到，如果给予足够的时间，两种H蛋白都支持神经侵袭和随后的临床神经系统体征的发展，表明疾病持续时间是主要的神经毒力决定因素。
The Morbillivirus hemagglutinin (H) protein mediates attachment to the target cell. To evaluate its contribution to canine distemper virus neurovirulence, we exchanged the H proteins of the wild-type strains 5804P and A75 and assessed the pathogenesis of the chimeric viruses in ferrets. Both strains are lethal to ferrets; however, 5804P causes a 2-week disease without neurological signs, whereas A75 is associated with a longer disease course and neurological involvement. We observed that both H proteins supported neuroinvasion and the subsequent development of clinical neurological signs if given enough time, demonstrating that disease duration is the main neurovirulence determinant.