Transient expression of the Arabidopsis thaliana callose synthase PMR4 increases penetration resistance to powdery mildew in barley.
Transient expression of the Arabidopsis thaliana callose synthase PMR4 increases penetration resistance to powdery mildew in barley.
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DOI：
10.4236/abb.2013.48106
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Voigt, C. A.
中科院分区：
文献类型：
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作者：
Blumke, A.;Somerville, S. C.;Voigt, C. A.
局部细胞壁增厚，所谓的乳突，是植物细胞渗透部位对真菌攻击的常见植物防御反应。乳突的主要成分是胼胝质，一种（1，3）-β-葡聚糖聚合物，有助于减缓或阻止真菌菌丝的入侵。在模式植物拟南芥（Arabidopsis thaliana）中，我们最近发现，编码胁迫诱导的愈伤组织合成酶的PMR 4（POWDERY MILDEW RESISTANT 4）的过表达导致完全的白粉病抗性。为了评估这些发现是否也可转移到单子叶作物，我们在大麦（Hordeum vulgare）幼苗的叶子中在35 S启动子的控制下瞬时表达PMR 4，随后用毒性白粉病Blumeria graminis f.大麦锈病菌融合的绿色荧光蛋白（GFP）的PMR 4允许成功转化大麦细胞的鉴定，这表明了增加的渗透阻力B。PMR 4-GFP以类似的模式定位在如A. thaliana，这表明双子叶植物和单子叶植物中可能存在类似的胼胝质合成酶转运机制。
Localized cell wall thickenings, so called papillae, are a common plant defense response to fungal attack at sites of penetration of the plant cell. The major constituent of papillae is callose, a (1,3)-beta-glucan polymer, which contributes to slowing or blocking the invading fungal hyphae. In the model plant Arabidopsis thaliana, we could recently show that the overexpression of PMR4(POWDERY MILDEW RESISTANT 4), which encodes a stress induced callose synthase, results in complete powdery mildew resistance. To evaluate if these findings are also transferable to monocot crops, we transiently expressed PMR4 under control of the 35S promoter in leaves of barley (Hordeum vulgare) seedlings, which were subsequently inoculated with the virulent powdery mildew Blumeria graminis f. sp. hordei. Fusion of the green fluorescent protein (GFP) to PMR4 allowed the identification of successfully transformed barley cells, which showed an increased penetration resistance to B. graminis compared to control cells that express only GFP.PMR4-GFP localized in a similar pattern at the site of attempted fungal penetration as observed inA. thaliana, which suggests that similar transport mechanisms of the callose synthase might exist in dicot and monocot plants.