Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer.
Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer.
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人类复制因子 C4（一种钳加载器）的水平与肿瘤进展相关并预测结直肠癌的预后
DOI：
10.1186/s12967-014-0320-0
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发表时间：
2014-11-19
影响因子：
7.4
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Xiang J;Fang L;Luo Y;Yang Z;Liao Y;Cui J;Huang M;Yang Z;Huang Y;Fan X;Wang H;Wang L;Peng J;Wang J
BackgroundHuman replication factor C4 (RFC4) is involved in DNA replication as a clamp loader and is aberrantly regulated across a range of cancers. The current study aimed to investigate the function of RFC4 in colorectal cancer (CRC).MethodsThe mRNA levels ofRFC4were assessed in 30 paired primary CRC tissues and matched normal colonic tissues by quantitative PCR. The protein expression levels ofRFC4were evaluated by western blotting (n = 16) and immunohistochemistry (IHC; n = 49), respectively. Clinicopathological features and survival data were correlated with the expression of RFC4 by IHC analysis in a tissue microarray comprising 331 surgically resected CRC. The impact of RFC4 on cell proliferation and the cell cycle was assessed using CRC cell lines.ResultsRFC4expression was significantly increased in CRC specimens as compared to adjacent normal colonic tissues (P<0.05). High levels of RFC4, determined on a tissue microarray, were significantly associated with differentiation, an advanced stage by the Tumor-Node-Metastasis (TNM) staging system, and a poor prognosis, as compared to low levels of expression (P<0.05). However, in multivariate analysis, RFC4 was not an independent predictor of poor survival for CRC. Invitrostudies, the loss of RFC4 suppressed CRC cell proliferation and induced S-phase cell cycle arrest.ConclusionRFC4is frequently overexpressed in CRC, and is associated with tumor progression and worse survival outcome. This might be attributed to the regulation of CRC cell proliferation and cell cycle arrest by RFC4.
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影响因子：
8.8
作者：
Fan, X-J;Wan, X-B;Huang, Y.;Cai, H-M;Fu, X-H;Yang, Z-L;Chen, D-K;Song, S-X;Wu, P-H;Liu, Q.;Wang, L.;Wang, J-P
通讯作者：
Wang, J-P
影响因子：
5.3
作者：
Krause, SA;Loupart, ML;Heck, MMS
通讯作者：
Heck, MMS
影响因子：
5.3
作者：
Overmeer, Rene M.;Gourdin, Audrey M.;Vermeulen, Wim
通讯作者：
Vermeulen, Wim
DOI：
10.1158/1078-0432.ccr-11-1431
发表时间：
2012-04-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Lockwood WW;Thu KL;Lin L;Pikor LA;Chari R;Lam WL;Beer DG
通讯作者：
Beer DG
DOI：
10.1158/1078-0432.ccr-08-2459
发表时间：
2009-03-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Erdogan E;Klee EW;Thompson EA;Fields AP
通讯作者：
Fields AP