Estrogen stimulates the human endometrium to express a factor(s) that promotes vascular smooth muscle cell migration as an early step in microvessel remodeling.
Estrogen stimulates the human endometrium to express a factor(s) that promotes vascular smooth muscle cell migration as an early step in microvessel remodeling.
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DOI：
10.1007/s12020-008-9128-0
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发表时间：
2009-02
期刊：
影响因子：
3.7
通讯作者：
Albrecht, Eugene D.
中科院分区：
文献类型：
--
作者：
Babischkin, Jeffery S.;Bonagura, Thomas W.;Udoff, Laurence C.;Vergara, Christine O.;Johnson, Harry W.;Atlas, Robert O.;Pepe, Gerald J.;Albrecht, Eugene D.
血管平滑肌细胞（VSMC）迁移是血管重塑的关键早期步骤;然而，对月经周期中人类子宫内膜中这一过程的调节知之甚少。在这项研究中，将人子宫内膜外植体与雌二醇和/或孕酮孵育，并将条件培养基（CM）应用于VSMC培养物，以检验雌激素和孕酮刺激子宫内膜细胞分泌促进VSMC迁移的因子的假设。子宫内膜外植体由高度组织化的腺体和基质组成。VSMC迁移在存在CM的情况下，来自月经周期增殖期获得并与雌二醇孵育24 h的人子宫内膜外植体的细胞（细胞在21 h/mm 2纤连蛋白包被的半透膜中迁移）的增殖能力约为对照组的3倍。（P < 0.001）和大于（P < 0.05）CM。因此，可以得出结论，雌激素刺激子宫内膜分泌一种促进VSMC迁移的因子，作为子宫内膜内血管重塑的早期步骤。
Vascular smooth muscle cell (VSMC) migration is a pivotal early step in blood vessel remodeling; however, very little is known about the regulation of this process in the human endometrium during the menstrual cycle. In this study, explants of human endometrium were incubated with estradiol and/or progesterone and the conditioned medium (CM) applied to cultures of VSMC to test the hypothesis that estrogen and progesterone stimulate endometrial cells to secrete a factor(s) that promotes VSMC migration. Endometrial explants were composed of highly organized glands and stroma. VSMC migration (cells migrated in 21 h/mm2 fibronectin-coated semipermeable membrane) in the presence of CM from human endometrial explants obtained in the proliferative phase of the menstrual cycle and incubated for 24 h with estradiol was approximately threefold greater (P < 0.001) than with medium alone and greater (P < 0.05) than with CM from explants treated with estradiol plus progesterone or progesterone. It is concluded, therefore, that estrogen stimulates endometrial secretion of a factor(s) that promotes VSMC migration as an early step in vessel remodeling within the endometrium.
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