Bovine serum albumin as the dominant form of dietary protein reduces subcutaneous fat mass, plasma leptin and plasma corticosterone in high fat-fed C57/BL6J mice
Bovine serum albumin as the dominant form of dietary protein reduces subcutaneous fat mass, plasma leptin and plasma corticosterone in high fat-fed C57/BL6J mice
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牛血清白蛋白作为膳食蛋白质的主要形式可降低高脂肪喂养的 C57/BL6J 小鼠的皮下脂肪量、血浆瘦素和血浆皮质酮
DOI：
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发表时间：
2015
影响因子：
3.6
通讯作者：
K. Nilaweera
中科院分区：
文献类型：
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作者：
Bettina McManus;R. Korpela;J. Speakman;J. Cryan;P. Cotter;K. Nilaweera
越来越多的证据表明，在人类和啮齿类动物的高脂肪喂养过程中，膳食蛋白质的来源会对体重增加和脂肪量产生影响。本研究考察了作为主要蛋白质来源的牛血清白蛋白（BSA）是否会改变能量平衡和与高脂肪饲养相关的肥胖。C57/BL6J小鼠分别饲喂10%能量来自脂肪、20%能量来自酪蛋白或45%能量来自脂肪、20%能量来自酪蛋白（HFD）或牛血清白蛋白（HFD+BSA）的饲粮，为期13周。与HFD相比，HFD+BSA对日能量消耗、运动活动和RER没有显著影响（P< 0.05），但增加了累积能量摄入和瘦质量百分比，降低了饲料效率和脂肪质量百分比（P< 0.05）。在皮下脂肪组织（SAT）中，与HFD相比，HFD+BSA饲粮提高了PPARA α (PPARA)、肉碱棕榈酰基转移酶1b （CPT1b）和解偶联蛋白3 (UCP3) mRNA水平，降低了瘦素mRNA水平（P< 0.05）。PPARA、CPT1b和UCP3的mRNA水平与SAT质量呈负相关（P< 0.05）， HFD+BSA小鼠的SAT质量较HFD对照组降低（P< 0.01）。各组间附睾脂肪量无显著差异。与HFD相比，HFD+BSA饮食使血浆瘦素和皮质酮水平正常化（P< 0.05）。瘦素水平差异与脂肪质量百分比相关（P< 0.01），皮质酮浓度变化与脂肪质量百分比无关（P< 0.05）。数据表明，HFD+BSA饮食通过SAT质量降低影响血浆瘦素水平，其中与β-氧化相关的基因mRNA水平升高，而血浆皮质酮水平的差异与脂肪质量减少无关。
Increasing evidence suggests that the source of dietary protein can have an impact on weight gain and fat mass during high-fat feeding in both humans and rodents. The present study examined whether dietary bovine serum albumin (BSA) as the dominant source of protein alters energy balance and adiposity associated with high-fat feeding. C57/BL6J mice were given a diet with 10 % of energy from fat and 20 % of energy from casein or a diet with 45 % of energy from fat and either 20 % of energy from casein (HFD) or BSA (HFD+BSA) for 13 weeks. The HFD+BSA diet did not significantly alter daily energy expenditure, locomotor activity and RER, but did increase cumulative energy intake and percentage of lean mass while reducing feed efficiency and percentage of fat mass when compared with the HFD (P< 0·05). In subcutaneous adipose tissue (SAT), the HFD+BSA diet increased the mRNA levels of PPARα (PPARA), carnitine palmitoyltransferase 1b (CPT1b) and uncoupling protein 3 (UCP3), but reduced the mRNA level of leptin when compared with the HFD (P< 0·05). The SAT mRNA levels of PPARA, CPT1b and UCP3 were negatively correlated (P< 0·05) with SAT mass, which was reduced in HFD+BSA mice compared with HFD controls (P< 0·01). No differences in epididymal fat mass existed between the groups. The HFD+BSA diet normalised plasma leptin and corticosterone levels compared with the HFD (P< 0·05). While differences in leptin levels were associated with the percentage of fat mass (P< 0·01), changes in corticosterone concentrations were independent of the percentage of fat mass (P< 0·05). The data suggest that the HFD+BSA diet influences plasma leptin levels via SAT mass reduction where mRNA levels of genes linked to β-oxidation were increased, whereas differences in plasma corticosterone levels were not related to fat mass reduction.
影响因子：
4.9
作者：
Marta Maria Swierczynska;Ivona Mateska;M. Peitzsch;S. R. Bornstein;Trian Chavakis;G. Eisenhofer;V. Lamounier-zepter;Suzanne Eaton
通讯作者：
Marta Maria Swierczynska;Ivona Mateska;M. Peitzsch;S. R. Bornstein;Trian Chavakis;G. Eisenhofer;V. Lamounier-zepter;Suzanne Eaton