NF-κB prevents TNF-α-induced apoptosis in an oligodendrocyte cell line
NF-κB prevents TNF-α-induced apoptosis in an oligodendrocyte cell line
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DOI：
10.1023/b:nere.0000029571.39497.56
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发表时间：
2004-08-01
影响因子：
4.4
通讯作者：
Takamatsu, K
中科院分区：
文献类型：
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作者：
Hamanoue, M;Yoshioka, A;Takamatsu, K
核因子κ b （nf - κ b）抑制中枢神经系统感觉神经元、海马神经元和纹状体神经元的凋亡。尽管一些凋亡刺激已被证明可以激活少突胶质细胞中的NF-kappaB，但NF-kappaB在这种细胞类型中的功能尚不清楚。在本研究中，我们将表达人NF-kappaB p50-或p65亚基的质粒引入大鼠少突胶质细胞前细胞系中枢胶质-4 （CG-4）中，并测定NF-kappaB功能对肿瘤坏死因子- α (tnf - α)诱导的细胞凋亡的影响。NF-kappaB的表达可显著抑制CG-4细胞凋亡，且p50的抑制作用强于p65。这种抗凋亡活性被NF-kappaB抑制剂ikappab - α抑制。这些结果表明，NF-kappaB是tnf诱导的少突胶质细胞凋亡的有效抑制剂。
Nuclear factor kappa beta (NF-kappaB) inhibits apoptosis in sensory, hippocampal, and striatal neurons of the central nervous system. Although several apoptotic stimuli have been shown to activate NF-kappaB in oligodendrocytes, the function of NF-kappaB in this cell type remains unknown. In this study, we introduced plasmids expressing either the p50- or p65-subunit of human NF-kappaB into Central Glia-4 (CG-4) - a rat oligodendrocyte precursor cell line - and determined the influence of NF-kappaB function on tumor necrosis factor-alpha (TNF-alpha)-induced apoptosis. Expression of NF-kappaB markedly prevented CG-4 apoptosis, with p50 being more effective than p65. This anti-apoptotic activity was repressed by IkappaB-alpha, an inhibitor of NF-kappaB. These results imply that NF-kappaB acts as a potent inhibitor of TNF-induced apoptosis in oligodendrocytes.