Olfactory Bulb Deep Short-Axon Cells Mediate Widespread Inhibition of Tufted Cell Apical Dendrites
Olfactory Bulb Deep Short-Axon Cells Mediate Widespread Inhibition of Tufted Cell Apical Dendrites
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DOI：
10.1523/jneurosci.2880-16.2016
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发表时间：
2017-02-01
影响因子：
5.3
通讯作者：
Urban, Nathaniel N.
中科院分区：
文献类型：
--
作者：
Burton, Shawn D.;LaRocca, Greg;Urban, Nathaniel N.
在嗅觉系统感觉加工的第一站--主嗅球（MOB）中，GABA能中间神经元信号塑造主神经元的活动，从而调节嗅觉。然而，缺乏已知的MOB中间神经元的选择性标记物，强烈阻碍了中间神经元功能的细胞类型选择性研究。在这里，我们确定了第一个选择性标记的肾小球层投射深短轴突细胞（GL-dSACs），并系统地研究了结构，丰度，内在生理学，前馈感觉输入，神经调节，突触输出，和功能作用的GL-dSACs在小鼠MOB电路。GL-dSAC位于内网状层，在那里它们将离心胆碱能输入与高度收敛的前馈感觉输入整合。GL-dSAC轴突树枝状广泛跨越肾小球层，以提供高度发散但选择性的输出到中间神经元和主簇状细胞上。因此，GL-dSAC能够响应于不同的感觉和自上而下的神经调节输入，在大范围的MOB中转移主要簇状细胞与二尖瓣细胞活性的平衡。
In the main olfactory bulb (MOB), the first station of sensory processing in the olfactory system, GABAergic interneuron signaling shapes principal neuron activity to regulate olfaction. However, a lack of known selective markers for MOB interneurons has strongly impeded cell-type-selective investigation of interneuron function. Here, we identify the first selective marker of glomerular layer-projecting deep short-axon cells (GL-dSACs) and investigate systematically the structure, abundance, intrinsic physiology, feedforward sensory input, neuromodulation, synaptic output, and functional role of GL-dSACs in the mouse MOB circuit. GL-dSACs are located in the internal plexiform layer, where they integrate centrifugal cholinergic input with highly convergent feedforward sensory input. GL-dSAC axons arborize extensively across the glomerular layer to provide highly divergent yet selective output onto interneurons and principal tufted cells. GL-dSACs are thus capable of shifting the balance of principal tufted versus mitral cell activity across large expanses of the MOB in response to diverse sensory and top-down neuromodulatory input.