Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny
Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny
复制标题
DOI：
10.3390/genes11070753
复制
发表时间：
2020-07-01
期刊：
影响因子：
3.5
通讯作者：
Varkonyi, Eszter Patakine
中科院分区：
文献类型：
--
作者：
Kaldy, Jeno;Mozsar, Attila;Varkonyi, Eszter Patakine
来自鲟鱼科和多齿鱼科的两个物种，俄罗斯鲟鱼（Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833；功能性四倍体）和美洲白鲟（Polyodon spathula, Walbaum 1792，功能性二倍体）进行了杂交。使用三只鲟鱼的卵和四只白鲟的精子重复进行杂交。孵化后 30 天，所有杂交家庭群体的存活率从 62% 到 74% 不等。这是这两个物种之间以及鲟科和多齿兽科成员之间的首次成功杂交。流式细胞术和染色体分析揭示了杂种中的两个倍性水平。杂交体的染色体数目范围分别为156-184和300-310，分别为“功能性”三倍体和“功能性”五倍体。杂交起源和倍性水平也通过微卫星分析得到证实。在杂交种中，背盾和腹盾的大小和数量与倍性水平以及母本和父本染色体组的计算比率相关。无论倍性水平如何，在三个杂交个体中都发现了额外的单倍体细胞谱系，这表明多精受精。尽管杂种的生长性能表现出很大的差异（平均值：1.2千克，标准差：0.55），但在集约饲养条件下，许多个体在一岁时就达到了约1千克的大小。
Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833; functional tetraploid) and American paddlefish (Polyodon spathula, Walbaum 1792, functional diploid) were hybridized. The hybridization was repeated using eggs from three sturgeon and sperm from four paddlefish individuals. Survival in all hybrid family groups ranged from 62% to 74% 30 days after hatching. This was the first successful hybridization between these two species and between members of the family Acipenseridae and Polyodontidae. Flow cytometry and chromosome analysis revealed two ploidy levels in hybrids. The chromosome numbers of the hybrids ranged between 156-184 and 300-310, in "functional" triploids and "functional" pentaploids, respectively. The hybrid origin and the ploidy levels were also confirmed by microsatellite analyses. In hybrids, the size and the number of dorsal and ventral scutes correlated with the ploidy levels as well as with the calculated ratio of the maternal and paternal chromosome sets. An extra haploid cell lineage was found in three hybrid individuals irrespective of the ploidy level, suggesting polyspermy. Although the growth performance showed high variance in hybrids (mean: 1.2 kg, SD: 0.55), many individuals reached a size of approximately 1 kg by the age of one year under intensive rearing conditions.