Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana
Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana
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DOI：
10.1093/dnares/10.6.239
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发表时间：
2003-12-31
期刊：
影响因子：
4.1
通讯作者：
Kikuchi, S
中科院分区：
文献类型：
--
作者：
Ooka, H;Satoh, K;Kikuchi, S
NAC结构域最初是从矮牵牛花NAM和拟南芥ATAF1、ATAF2和CUC2的一致序列中鉴定出来的。含有NAC结构域的基因（NAC家族基因）是植物特异性转录调控因子，在不同发育阶段和组织中均有表达。我们对单子叶水稻（Oryza sativa）和双子叶拟南芥（Arabidopsis thaliana） NAC家族基因进行了综合分析。在拟南芥（A. sativa）全长cDNA数据集中（28,469个克隆）发现了75个预测NAC蛋白，在拟南芥基因组推定基因（28,581个序列）中发现了105个预测NAC蛋白。根据序列相似性将预测和已知NAC家族蛋白的NAC结构域划分为2组和18个亚组。唾液拟南芥与拟南芥NAC结构域的氨基酸序列存在一定差异。此外，我们在预测的NAC蛋白的c端区发现了13个来自转录激活区的共同序列基序。这些基序可能与NAC结构域结构有关。我们根据我们的结果和已发表的数据讨论了NAC家族蛋白的结构和功能之间的关系。本研究结果将有助于进一步分析NAC家族基因的功能。
The NAC domain was originally characterized from consensus sequences from petunia NAM and from Arabidopsis ATAF1, ATAF2, and CUC2. Genes containing the NAC domain (NAC family genes) are plant-specific transcriptional regulators and are expressed in various developmental stages and tissues. We performed a comprehensive analysis of NAC family genes in Oryza sativa (a monocot) and Arabidopsis thaliana (a dicot). We found 75 predicted NAC proteins in full-length cDNA data sets of O. sativa (28,469 clones) and 105 in putative genes (28,581 sequences) from the A. thaliana genome. NAC domains from both predicted and known NAC family proteins were classified into two groups and 18 subgroups by sequence similarity. There were a few differences in amino acid sequences in the NAC domains between O. saliva and A. thaliana. In addition, we found 13 common sequence motifs from transcriptional activation regions in the C-terminal regions of predicted NAC proteins. These motifs probably diverged having correlations with NAC domain structures. We discuss the relationship between the structure and function of the NAC family proteins in light of our results and the published data. Our results will aid further functional analysis of NAC family genes.