UV-induced protein alterations and lipid oxidation in erythrocyte membranes.
UV-induced protein alterations and lipid oxidation in erythrocyte membranes.
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紫外线诱导红细胞膜中的蛋白质改变和脂质氧化。
DOI：
10.1111/j.1751-1097.1990.tb08684.x
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发表时间：
1990
影响因子：
3.3
通讯作者：
Kochevar,IE
中科院分区：
文献类型：
--
作者：
Kochevar,IE
皮肤中的某些紫外线辐射效应可能是由细胞膜的初级光化学变化引起的。我们对红细胞膜进行了研究，以确定蛋白质变化和脂质氧化的紫外光强度和波长依赖性。聚丙烯酰胺/琼脂糖凝胶电泳法检测到高分子量蛋白质(>；200000 Da)。在λ=2 5 0-380 nm辐射下，血影蛋白比其他膜蛋白下降得更快。氮气吹扫可抑制紫外光诱导的血影蛋白含量下降60%，并能更大程度地降低交联度。超氧化物歧化酶、过氧化氢酶或叠氮钠不能抑制血影蛋白的减少。280 nm照射效果最好，265和297 nm次之，254和313 nm效果不明显。先前在280 nm处的照射没有使膜对随后的313 nm处的照射敏感，这表明色氨酸的光解产物不参与。用硫代巴比妥酸比色法测定脂质光氧化，并在比失去血影蛋白所需的更高的紫外线辐射强度下诱导脂质光氧化。这些结果表明，UV辐射对细胞膜的主要影响是蛋白质的变化，并表明色氨酸是这些变化的主要发色团。
Certain ultraviolet radiation‐induced effects in skin may result from primary photochemical alterations in cell membranes. We have studied isolated erythrocyte membranes in order to determine the UV‐fiuence and wavelength dependence for protein alterations and lipid oxidation. Protein crosslinking was detected as high molecular weight protein (> 200 000 Da) on polyacrylamide/agarose gel electrophoresis. Spectrin decreased more rapidly than the other membrane proteins upon exposure to λ= 250–380 nm radiation. Nitrogen‐purging inhibited the UV‐induced decrease in spectrin by 60% and decreased crosslinking to an even greater degree. The decrease in spectrin was not inhibited by superoxide dismutase, catalase, or sodium azide. Radiation at 280 nm was most effective for spectrin loss, 265 and 297 nm were less effective and 254 and 313 nm were not effective. Prior irradiation at 280 nm did not sensitize the membranes to subsequent irradiation at 313 nm indicating that photodecomposition products of tryptophan are not involved. Lipid photooxidation was measured with the thiobarbituric acid assay and was induced at higher fluences of UV radiations than those required for loss of spectrin. These results indicate that the major effects of UV radiation on cell membranes are alterations of proteins and suggest that tryptophan is the major chromophore for these alterations.