Meiotic proteins Bqt1 and Bqt2 tether telomeres to form the bouquet arrangement of chromosomes
Meiotic proteins Bqt1 and Bqt2 tether telomeres to form the bouquet arrangement of chromosomes
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DOI：
10.1016/j.cell.2006.01.048
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发表时间：
2006-04-07
期刊：
影响因子：
64.5
通讯作者：
Hiraoka, Y
中科院分区：
文献类型：
--
作者：
Chikashige, Y;Tsutsumi, C;Hiraoka, Y
在许多生物体中，减数分裂染色体被捆绑在它们的端粒上，形成“花束”的排列。花束的形成在同源染色体配对过程中起着重要的作用。由于分裂酵母的减数分裂端粒聚集是对交配信息素信号的响应，我们在交配信息素信号诱导的基因中寻找花束形成的必要因素。这项全基因组搜索确定了两种蛋白质，Bqt1和Bqt2，它们连接端粒和纺锤极体（SPB；真菌中的中心体等量物）。单独的Bqt1和Bqt2都不能作为连接物，但是这两种蛋白一起形成了Rap1（一种端粒蛋白）和Sad1（一种SPB蛋白）之间的桥梁。值得注意的是，当Bqt1和Bqt2在有丝分裂细胞中异位表达时，它们也形成了Rap1和Sad1之间的桥梁。因此，包括Bqt1和Bqt2的复合体对于连接端粒和SPB至关重要。
In many organisms, meiotic chromosomes are bundled at their telomeres to form a "bouquet" arrangement. The bouquet formation plays an important role in homologous chromosome pairing and therefore progression of meiosis. As meiotic telomere clustering occurs in response to mating pheromone signaling in fission yeast, we looked for factors essential for bouquet formation among genes induced under mating pheromone signaling. This genome-wide search identified two proteins, Bqt1 and Bqt2, that connect telomeres to the spindle-pole body (SPB; the centrosome equivalent in fungi). Neither Bqt1 nor Bqt2 alone functions as a connector, but together the two proteins form a bridge between Rap1 (a telomere protein) and Sad1 (an SPB protein). Significantly, when both Bqt1 and Bqt2 are ectopically expressed in mitotic cells, they also form a bridge between Rap1 and Sad1. Thus, a complex including Bqt1 and Bqt2 is essential for connecting telomeres to the SPB.