The role of Rhipicephalus sanguineus sensu lato saliva in the dissemination of Rickettsia conorii in C3H/HeJ mice.
The role of Rhipicephalus sanguineus sensu lato saliva in the dissemination of Rickettsia conorii in C3H/HeJ mice.
复制标题
血扇头象唾液在 C3H/HeJ 小鼠康氏立克次体传播中的作用。
DOI：
10.1111/mve.12118
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发表时间：
2015
影响因子：
1.9
通讯作者：
Walker,DH
中科院分区：
文献类型：
--
作者：
Milhano,N;Saito,TB;Bechelli,J;Fang,R;Vilhena,M;DESousa,R;Walker,DH
已开发出用于研究立克次体发病机制的动物模型。然而，为了了解立克次体通过蜱虫叮咬传播的自然途径中发生的情况，应在这些模型中考虑蜱虫唾液的作用。为了解决这个问题，我们分析了蜱唾液在鼠类宿主中康氏立克次体（立克次体目：立克次体科）传播中的作用，通过皮内（i.d.）用立克次体接种两组易感C3H/HeJ小鼠，并用若虫Rhipicephalus sanguineussensu lato（Ixodida：Ixodidae）蜱感染一组。分别通过实时定量聚合酶链式反应 (PCR) 和实时逆转录酶 PCR 对 C3H/HeJ 肺样本中的细菌负荷和白介素-1β (IL-1β)、IL-10 和 NF-κB mRNA 水平进行定量。检查肺组织学以评估感染的病理表现。两组小鼠肺部细菌负荷没有观察到统计学上的显着差异；然而，IL-1β和NF-κB水平存在统计学上的显着差异，这两种水平在接种立克次体但未感染蜱的组中均较高。两组动物的肺部组织学均显示炎症细胞浸润。总的来说，这项研究表明 i.d. R的接种。 C3H/HeJ 小鼠肺部锥孔引起的感染和蜱唾液抑制促炎作用。
Animal models have been developed for the study of rickettsial pathogenesis. However, to understand what occurs during the natural route of rickettsial transmission via the tick bite, the role of tick saliva should be considered in these models. To address this, we analysed the role of tick saliva in the transmission ofRickettsia conorii(Rickettsiales: Rickettsiaceae) in a murine host by intradermally (i.d.) inoculating two groups of susceptible C3H/HeJ mice with thisRickettsia, and infesting one group with nymphalRhipicephalus sanguineussensu lato(Ixodida: Ixodidae) ticks. Quantification of bacterial loads and mRNA levels of interleukin‐1β (IL‐1β), IL‐10 and NF‐κB was performed in C3H/HeJ lung samples by real‐time quantitative polymerase chain reaction (PCR) and real‐time reverse transcriptase PCR, respectively. Lung histology was examined to evaluate the pathological manifestations of infection. No statistically significant difference in bacterial load in the lungs of mice was observed between these two groups; however, a statistically significant difference was observed in levels of IL‐1β and NF‐κB, both of which were higher in the group inoculated with rickettsiae but not infected with ticks. Lung histology in both groups of animals revealed infiltration of inflammatory cells. Overall, this study showed that i.d. inoculation ofR. conoriicaused infection in the lungs of C3H/HeJ mice and tick saliva inhibited proinflammatory effects.