THE MURINE LIM-KINASE GENE (LIMK) ENCODES A NOVEL SERINE THREONINE KINASE EXPRESSED PREDOMINANTLY IN TROPHOBLAST GIANT-CELLS AND THE DEVELOPING NERVOUS-SYSTEM
THE MURINE LIM-KINASE GENE (LIMK) ENCODES A NOVEL SERINE THREONINE KINASE EXPRESSED PREDOMINANTLY IN TROPHOBLAST GIANT-CELLS AND THE DEVELOPING NERVOUS-SYSTEM
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DOI：
10.1016/0925-4773(95)00400-u
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发表时间：
1995-08-01
影响因子：
2.6
通讯作者：
ROBERTSON, EJ
中科院分区：
文献类型：
--
作者：
CHENG, AK;ROBERTSON, EJ
在整个脊椎动物胚胎发生过程中，膜结合和细胞内蛋白激酶控制着协调细胞生长和分化所必需的基本决定。在这里，我们的特点limk，一种新的蛋白激酶丝氨酸苏氨酸底物特异性，也包含两个LIM结构域。我们使用北方印迹和原位杂交技术，以确定其在小鼠发育早期的表达模式。在7.5到8.5 d.p.c.之间，Limk在胚胎内的三个广泛区域中表达，即预期前脑和中脑区域的神经外胚层、心脏中胚层和新形成的定形内胚层衍生物前肠和后肠。10.0 d.p.c.时Limk在发育中的前脑和中脑的腹内侧区域保持显著表达，在后肠中继续表达。在成年人中，limk在大脑中的表达最为突出。此外，我们还发现limk在妊娠后4.5天开始在滋养层巨细胞中表达最丰富。此外，高水平的limk表达与从二倍体祖细胞明显形成巨细胞相关，表明limk参与这种高度特化的胚外细胞类型的分化。
Throughout vertebrate embryogenesis, membrane bound and intracellular protein kinases govern the fundamental decisions necessary for coordinated cell growth and differentiation. Here we have characterized limk, a novel protein kinase with serine threonine substrate specificity which also contains two LIM domains. We used Northern blot and in situ hybridization techniques to determine its pattern of expression in early mouse development. Between 7.5 and 8.5 d.p.c., limk is expressed in three broad domains within the embryo, the neuroectodermal of the prospective forebrain and mid-brain regions, the cardiac mesoderm, and the newly formed definitive endodermal derivatives the foregut and hindgut. By 10.0 d.p.c. limk remains prominently expressed in the ventromedial regions of the developing forebrain and midbrain, with continued expression in the hindgut. In adults limk is expressed most prominently in the brain. Additionally we have shown that limk is most abundantly expressed in the trophoblast giant cells, from 4.5 d.p.c onwards. Moreover, high levels of limk expression is associated with the overt formation of giant cells from diploid progenitors, suggesting an involvement for limk in the differentiation of this highly specialized extra-embryonic cell type.