Alterations in the gut microbiome and metabolism with coronary artery disease severity
Alterations in the gut microbiome and metabolism with coronary artery disease severity
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肠道微生物组和代谢随冠状动脉疾病严重程度的变化
DOI：
10.1186/s40168-019-0683-9
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发表时间：
2019-04-26
期刊：
影响因子：
15.5
通讯作者：
Zhang, Chenhong
中科院分区：
文献类型：
--
作者：
Liu, Honghong;Chen, Xi;Zhang, Chenhong
背景冠状动脉疾病（CAD）与不同人群肠道菌群的改变有关。肠道微生物衍生的代谢产物已被提议作为主要不良心脏事件的标志物。然而，肠道微生物组和CAD病理生理学的不同阶段之间的关系仍然有待建立一个系统的study.ResultsBased多组学分析（16 S rRNA基因和代谢组学的V3-V4区域的测序）的161例CAD患者和40名健康对照，我们发现，肠道微生物和代谢物的组成与CAD的严重程度显着变化。共鉴定出29个代谢物模块，分别与CAD表型呈正相关或负相关，其中罗斯拜瑞氏菌属、克雷伯氏菌属、IV型梭菌属和瘤胃球菌科为代表的细菌共丰度群（CAG）在CAD不同阶段具有特征性变化。结果表明，某些细菌可能通过调节宿主的代谢途径，如牛磺酸、鞘脂和神经酰胺以及苯代谢而影响动脉粥样硬化。此外，疾病分类器的基础上的微生物和代谢产物的差异水平的构建，以区别于对照组的情况下，甚至能够区分稳定型冠状动脉疾病从急性冠状动脉syndrome accurately.ConclusionOverall，肠道微生物群落的组成和功能不同的健康对照不同的冠状动脉疾病亚型。我们的研究确定了肠道微生物群特征与循环代谢产物之间的关系，为未来旨在了解宿主-肠道微生物群在动脉粥样硬化发病机制中相互作用的研究提供了新的方向。
BackgroundCoronary artery disease (CAD) is associated with gut microbiota alterations in different populations. Gut microbe-derived metabolites have been proposed as markers of major adverse cardiac events. However, the relationship between the gut microbiome and the different stages of CAD pathophysiology remains to be established by a systematic study.ResultsBased on multi-omic analyses (sequencing of the V3-V4 regions of the 16S rRNA gene and metabolomics) of 161 CAD patients and 40 healthy controls, we found that the composition of both the gut microbiota and metabolites changed significantly with CAD severity. We identified 29 metabolite modules that were separately classified as being positively or negatively correlated with CAD phenotypes, and the bacterial co-abundance group (CAG) with characteristic changes at different stages of CAD was represented byRoseburia,Klebsiella,Clostridium IVandRuminococcaceae. The result revealed that certain bacteria might affect atherosclerosis by modulating the metabolic pathways of the host, such as taurine, sphingolipid and ceramide, and benzene metabolism. Moreover, a disease classifier based on differential levels of microbes and metabolites was constructed to discriminate cases from controls and was even able to distinguish stable coronary artery disease from acute coronary syndrome accurately.ConclusionOverall, the composition and functions of the gut microbial community differed from healthy controls to diverse coronary artery disease subtypes. Our study identified the relationships between the features of the gut microbiota and circulating metabolites, providing a new direction for future studies aiming to understand the host–gut microbiota interplay in atherosclerotic pathogenesis.