RETROVIRAL DNA INTEGRATION - STRUCTURE OF AN INTEGRATION INTERMEDIATE
RETROVIRAL DNA INTEGRATION - STRUCTURE OF AN INTEGRATION INTERMEDIATE
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DOI：
10.1016/0092-8674(88)90071-2
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发表时间：
1988-08-12
期刊：
影响因子：
64.5
通讯作者：
MIZUUCHI, K
中科院分区：
文献类型：
--
作者：
FUJIWARA, T;MIZUUCHI, K
逆转录病毒DNA整合中假定的DNA中间体的结构在与外源添加的靶DNA的无细胞反应中进行了研究。由含有线性病毒DNA的莫洛尼鼠白血病病毒（Mo-MLV）的病毒核心颗粒制备的产物具有与在噬菌体Mu转座期间观察到的整合机制类似的整合机制一致的结构。在该中间体中，LTR序列的3“末端与靶DNA连接，而病毒DNA的5”末端保持未连接。中间体中LTR序列的5“末端与未整合的线性双链病毒DNA中发现的那些完全相同。该结果表明，线性形式的Mo-MLV DNA可以直接整合而无需事先环化。
The structure of a presumptive DNA intermediate in the integration of retrovial DNA was studied in a cell-free reaction with exogenously added target DNA. The product made by viral core particles of Moloney murine leukemia virus (Mo-MLV) containing linear viral DNA has a structure consistent with an integration mechanism similar to that observed during bacteriophage Mu transposition. In this intermediate, the 3'' ends of the LTR sequences are joined to the target DNA, while the 5'' ends of the viral DNA remain unjoined. The 5'' ends of the LTR sequences in the intermediate are exactly the same as those found in the unintegrated linear double-stranded viral DNA. This result demonstrates that the linear form of Mo-MLV DNA can integrate directly without prior circularization.