Phylogeny of Methanopyrus kandleri based on methyl coenzyme M reductase operons.
Phylogeny of Methanopyrus kandleri based on methyl coenzyme M reductase operons.
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基于甲基辅酶 M 还原酶操纵子的 Methanopyrus kandleri 系统发育。
DOI：
10.1099/00207713-46-4-1170
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
J. N. Reeve
中科院分区：
文献类型：
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作者：
Jork Nolling;Amy Elfner;John R. Palmer;V. J. Steigerwald;T. Pihl;James A. Lake;J. N. Reeve
克隆并测序了超嗜热菌Methanopyrus kandleri中编码甲基辅酶M还原酶（MR）的mcrBDCGA操纵子。对现有的9个MR序列进行系统发育分析的结果支持M. Kandleri是单独的产甲烷菌谱系。与其他产甲烷菌一样，M. kandleri mcr操纵子直接位于mtrE基因的上游，mtrE基因是编码N5-甲基四氢甲烷蝶呤：辅酶M甲基转移酶的操纵子中的启动子近端基因，该甲基转移酶催化甲烷生成中MR催化反应之前的步骤。与其他产甲烷菌和极端嗜热菌不同，CG二核苷酸和含CG密码子在M.坎德莱里DNA MR亚基编码基因之前的序列与核糖体结合位点一致，表明mRNA-rRNA碱基配对仍然可以在100摄氏度以上的温度下生长的细胞中指导翻译起始。
The mcrBDCGA operon that encodes methyl coenzyme M reductase (MR) in the hyperthermophile Methanopyrus kandleri was cloned and sequenced. The results of a phylogenetic analysis of the nine MR sequences now available support the position that M. kandleri is a separate methanogen lineage. As in other methanogens, the M. kandleri mcr operon is located immediately upstream of the mtrE gene, the promoter-proximal gene in an operon that encodes the N5-methyltetrahydromethanopterin:coenzyme M methyltransferase that catalyzes the step preceding the MR-catalyzed reaction in methanogenesis. In contrast to other methanogens and hyperthermophilic members of the Archaea, CG dinucleotides and CG-containing codons occur frequently in M. kandleri DNA. The MR subunit-encoding genes are preceded by sequences consistent with ribosome binding sites, indicating that mRNA-rRNA base pairing can still direct translation initiation in cells growing at temperatures above 100 degrees C.