Responses of neurons containing VGLUT3/nNOS-cGMP in the rVLM to cardiac stimulation
Responses of neurons containing VGLUT3/nNOS-cGMP in the rVLM to cardiac stimulation
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DOI：
10.1097/01.wnr.0000203351.06881.54
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发表时间：
2006-02-27
期刊：
影响因子：
1.7
通讯作者：
Longhurst, JC
中科院分区：
文献类型：
--
作者：
Guo, ZL;Longhurst, JC
延髓头端腹外侧区谷氨酸能神经元对心交感传入神经刺激的反应尚未明确。一氧化氮影响谷氨酸的神经功能。我们研究了心脏刺激后延髓吻端腹外侧区囊泡谷氨酸转运体3、c-Fos与神经元型一氧化氮合酶/可溶性鸟苷酸环化酶之间的关系。在有压力神经支配和迷走神经切断的麻醉猫中，心外膜应用缓激肽，而不是它的载体，引起升压反应。在延髓吻侧腹外侧区，缓激肽处理组(n=6；P<0.05)内含有c-Fos或c-Fos和神经元型一氧化氮合酶的囊泡状谷氨酸转运体3神经元多于对照组(n=4)。刺激缓激肽后，神经元型一氧化氮合酶、可溶性鸟苷酸环化酶和c-Fos或囊泡型谷氨酸转运体3共定位。结果表明，心脏刺激可激活延髓头端腹外侧区谷氨酸能神经元，这可能受一氧化氮通过cGMP的影响。
Responses of glutamatergic neurons in the rostral ventrolateral medulla to stimulation of cardiac sympathetic afferents have not been defined. Nitric oxide influences neural function of glutamate. We evaluated the relationship between vesicular glutamate transporter 3, c-Fos and neuronal nitric oxide synthase/soluble guanylyl cyclase in the rostral ventrolateral medulla following cardiac stimulation. In anesthetized cats with barodenervation and vagotomy, epicardial application of bradykinin, but not its vehicle, caused pressor responses. More vesicular glutamate transporter 3-containing neurons colocalized with c-Fos or c-Fos and neuronal nitric oxide synthase in the rostral ventrolateral medulla in bradykinin-treated cats (n=6; P < 0.05) than in control animals (n=4). Colocalization of neuronal nitric oxide synthase, soluble guanylyl cyclase and c-Fos or vesicular glutamate transporter 3 was noted following bradykinin stimulation. Findings indicate activation of rostral ventrolateral medulla glutamatergic neurons by cardiac stimulation, which may be influenced by nitric oxide via cGMP.