Alpha-subunit of the Chloroplast ATP Synthase of Tomato Reinforces the Resistance to Grey Mold and Broad-spectrum Resistance in Transgenic Tobacco
Alpha-subunit of the Chloroplast ATP Synthase of Tomato Reinforces the Resistance to Grey Mold and Broad-spectrum Resistance in Transgenic Tobacco
复制标题
番茄叶绿体 ATP 合酶的 α 亚基增强转基因烟草对灰霉病的抗性和广谱抗性
作者：
Chao Gong;Mo-zhen Cheng;Jing-fu Li;Hong-yu Chen;Zhen-zhu Zhang;Hao-nan Qi;Yao Zhang;Jiayin Liu;Xiu-ling Chen;Ao-xue Wang
叶绿体ATP合成酶（cpATPase）是光合作用中ATP合成的主要酶。我们的前期研究表明，cpATPase CF 1 α亚基（AtpA）是玫瑰粘帚霉（Clonostachys rosea）的一个关键蛋白。rosea）诱导的对真菌灰葡萄孢（B.番茄）。在此，我们发现番茄的cpATPase CF 1 α亚基基因（atpA）的表达水平被B上调。cinerea和C.玫瑰色。然后分离番茄atpA基因，获得转基因烟草品系。与未转化植株相比，atpA过表达烟草对B的抗性增强。灰霉病具有降低发病率、防御相关过敏反应（HR）样反应、平衡活性氧、减轻叶绿体超微结构损伤、提高ATP含量和cpATPase活性、增强碳代谢、光合作用和防御相关基因表达等特点。转基因烟草接种B后，Ca2+外排增加，H+外排稳定。灰叶此外，atpA基因的过表达赋予增强的耐盐性和抗真菌Cladosporiumfulvum。因此，cpATP酶的α亚基是一个关键的调节器，其将信号传导与细胞氧化还原稳态、ATP生物合成和抗性性状的基因表达联系起来，以调节对病原体感染的免疫力，从而在植物中提供广谱抗性。
Chloroplast ATP synthase (cpATPase) is responsible for ATP production during photosynthesis. Our previous studies showed the cpATPase CF1 alpha subunit (AtpA) is a key protein involved in Clonostachys rosea (C. rosea)-induced resistance to the fungus Botrytis cinerea (B. cinerea) in tomato. Here, we show the expression level of tomato’s cpATPase CF1 alpha subunit gene (atpA) was up-regulated by B. cinerea and C. rosea. The tomato atpA gene was then isolated, and transgenic tobacco lines were obtained. Compared with untransformed plants, the atpA-overexpressing tobacco showed an increased resistance to B. cinerea, characterized by reduced disease incidence, defense-associated hypersensitive response (HR)-like reactions, balanced reactive oxygen species, alleviated damage to chloroplast ultra-structure of leaf cell, elevated levels of ATP content and cpATPase activity, enhanced expression of carbon metabolism-, photosynthesis-, and defense-related genes. Incremental Ca2+ efflux and steady H+ efflux were observed in transgenic tobacco after their inoculation with B. cinerea. Additionally, overexpression of atpA gene conferred enhanced tolerance to salinity and resistance to the fungus Cladosporium fulvum. Thus, the α subunit of cpATPase is a key regulator that links signaling to cellular redox homeostasis, ATP biosynthesis, and gene expression of resistance traits to modulate immunity to pathogen infection, in the process providing broad-spectrum resistance in plants.