Mycobacterium avium uses apoptotic macrophages as tools for spreading
Mycobacterium avium uses apoptotic macrophages as tools for spreading
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DOI：
10.1016/j.micpath.2010.12.004
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发表时间：
2011-02-01
影响因子：
3.8
通讯作者：
Bermudez, Luiz E.
中科院分区：
文献类型：
--
作者：
Early, Julie;Fischer, Kay;Bermudez, Luiz E.
背景：鸟分枝杆菌（MAC）在巨噬细胞中生存和复制，并在免疫功能低下的个体中引起传播性疾病。作为控制疾病的宿主反应，许多巨噬细胞在MAC感染后几天变得凋亡。在这项研究中，我们假设MAC可以生存自噬和凋亡的巨噬细胞和spreaded.Methods：电子，延时录像，荧光显微镜。ELISA法和TUNEL法检测细胞凋亡。结果：凋亡的巨噬细胞主要携带有活性的MAC。一旦细胞凋亡被触发，MAC就会逃离空泡和巨噬细胞，使细菌在传播过程中自由感染附近的巨噬细胞。此外，一些MAC物质将具有凋亡小体，并在凋亡小体摄取后在健康巨噬细胞中释放。由于自噬先于细胞凋亡，它被建立，热杀死MAC，和可行的MAC诱导自噬在巨噬细胞以类似的速度，但MAC仍然survives.Conclusion：MAC传播从细胞到细胞是由巨噬细胞的企图杀死细菌，发生凋亡。(C)2010爱思唯尔有限公司版权所有。
Background: Mycobacterium avium (MAC) lives and replicates in macrophages and causes disseminated disease in immunocompromised individuals. As a host response to control disease, many macrophages become apoptotic a few days after MAC infection. In this study, we hypothesized that MAC can survive autophagic and apoptotic macrophages and spread.Methods: Electron, time-lapse video, fluorescence microscopy. Apoptosis was determined by ELISA and TUNEL assays. Autophagy was seen by migration of LC3-1.Results: Apoptotic macrophages harbor chiefly viable MAC. MAC escapes both the vacuole and the macrophage once apoptosis is triggered, leaving the bacteria free to infect nearby macrophages in the process of spreading. In addition, some MAC species will have apoptotic bodies and are released in healthy macrophages following apoptotic body ingestion. Because autophagy precedes apoptosis, it was established that heat-killed MAC, and viable MAC induces autophagy in macrophages at similar rates, but MAC still survives.Conclusion: MAC spreading from cell-to-cell is triggered by the macrophage's attempt to kill the bacterium, undergoing apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.