Prostaglandins E1 and E2 interact with prostaglandin F2alpha to regulate initiation of DNA replication and cell division in swiss 3T3 cells.
Prostaglandins E1 and E2 interact with prostaglandin F2alpha to regulate initiation of DNA replication and cell division in swiss 3T3 cells.
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前列腺素 E1 和 E2 与前列腺素 F2α 相互作用，调节瑞士 3T3 细胞中 DNA 复制和细胞分裂的起始。
DOI：
10.1073/pnas.79.16.4992
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发表时间：
1982
影响因子：
11.1
通讯作者：
L. Jiménez de Asúa
中科院分区：
文献类型：
--
作者：
A. Otto;M. Nilsen;B. D. Boss;M. Ulrich;L. Jiménez de Asúa
将 2-1,000 ng/ml 的前列腺素 (PG) E1 或 E2 添加到 Swiss 3T3 细胞的静止培养物中，可协同提高单独使用 PGF2 α 或与胰岛素一起刺激的 DNA 复制起始速率。 PGD2 和 PGF1 α 均对 PGF2 α 没有任何影响。当添加 PGF2 α 后 8 或 15 小时添加 PGE1 或 PGE2 时，进入 S 期的速率也会增加。然而，将PGE1和PGE2与PGF2α一起添加并没有进一步增强单独使用PGE1或PGE2观察到的协同效应。在刺激 2-脱氧葡萄糖摄取中也观察到了协同效应，但在细胞内 cAMP 水平的早期变化中没有观察到协同效应。这些结果可能与理解伤口愈合中成纤维细胞增殖的控制有关，并可能为致癌转化提供替代机制。
Prostaglandin (PG) E1 or E2 added at 2-1,000 ng/ml to quiescent cultures of Swiss 3T3 cells synergistically enhanced the rate of initiation of DNA replication stimulated by PGF2 alpha alone or with insulin. Neither PGD2 nor PGF1 alpha had any effect with PGF2 alpha. An increase in the rate of entry into S phase also occurred when PGE1 or PGE2 was added 8 or 15 hr after addition of PGF2 alpha. However, adding PGE1 and PGE2 together with PGF2 alpha did not further enhance the synergistic effect observed with PGE1 or PGE2 separately. The synergistic effect was also observed in stimulation of 2-deoxyglucose uptake but not in early changes of intracellular levels of cAMP. These results may be relevant in understanding the control of fibroblastic proliferation in wound healing and may provide an alternative mechanism for oncogenic transformation.