ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity.
ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity.
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DOI：
10.3389/fpls.2022.979956
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
盐生植物Karelinia caspia不仅具有饲料和药用价值，还可以修复盐碱地。我们前期的研究表明，盐腺分泌盐是卡斯皮亚在高盐环境下的主要适应策略之一。然而，里海岬的ROS清除、离子稳态和光合特性对高盐度的反应仍不清楚。在这里，在 100–400 mM NaCl 作用下的 K. caspia 中测试了与 ROS 清除和离子运输相关的生理生化反应和基因表达 7 天。结果显示，抗氧化酶（SOD、APX）活性和非酶抗氧化剂（绿原酸、α-生育酚、类黄酮、多胺）含量均显着增强，同时上调相关酶和非酶抗氧化合成基因（KcCu/Zn-SOD、KcAPX6、KcHCT、KcHPT1、Kcγ-TMT、KcF3H、KcSAMS 和KcSMS）随 NaCl 浓度的增加而表达。这些反应有利于去除过量的 ROS，以维持 H2O2 和 O2− 的稳定水平，而不会导致凯斯皮亚对高盐反应中的脂质过氧化。同时，KcSOS1/2/3、KcNHX1 和 KcAVP 表达的上调与 K. caspia 中 Na+ 进入液泡或通过盐腺排泄有关。值得注意的是，盐可以提高PSII促进净光合速率的功能，有助于在高盐度下正常生长。总体而言，研究结果表明，ROS 清除系统和 Na+/K+ 运输协同促进氧化还原平衡、离子稳态和 PSII 功能增强，从而赋予高盐耐受性。
The halophyte Karelinia caspia has not only fodder and medical value but also can remediate saline-alkali soils. Our previous study showed that salt-secreting by salt glands is one of main adaptive strategies of K. caspia under high salinity. However, ROS scavenging, ion homeostasis, and photosynthetic characteristics responses to high salinity remain unclear in K. caspia. Here, physio-biochemical responses and gene expression associated with ROS scavenging and ions transport were tested in K. caspia subjected to 100–400 mM NaCl for 7 days. Results showed that both antioxidant enzymes (SOD, APX) activities and non-enzymatic antioxidants (chlorogenic acid, α-tocopherol, flavonoids, polyamines) contents were significantly enhanced, accompanied by up-regulating the related enzyme and non-enzymatic antioxidant synthesis gene (KcCu/Zn-SOD, KcAPX6, KcHCT, KcHPT1, Kcγ-TMT, KcF3H, KcSAMS and KcSMS) expression with increasing concentrations of NaCl. These responses are beneficial for removing excess ROS to maintain a stable level of H2O2 and O2− without lipid peroxidation in the K. caspia response to high salt. Meanwhile, up-regulating expression of KcSOS1/2/3, KcNHX1, and KcAVP was linked to Na+ compartmentalization into vacuoles or excretion through salt glands in K. caspia. Notably, salt can improve the function of PSII that facilitate net photosynthetic rates, which is helpful to growing normally in high saline. Overall, the findings suggested that ROS scavenging systems and Na+/K+ transport synergistically contributed to redox equilibrium, ion homeostasis, and the enhancement of PSII function, thereby conferring high salt tolerance.
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DOI：
10.1007/s12298-022-01134-2
发表时间：
2022-01
期刊：
Physiology and molecular biology of plants : an international journal of functional plant biology
影响因子：
--
作者：
Chowaniec K;Rola K
通讯作者：
Rola K
影响因子：
6.9
作者：
Fryer, MJ;Oxborough, K;Baker, NR
通讯作者：
Baker, NR
影响因子：
1.9
作者：
Bueno, Milagros;Lucia Lendinez, Maria;del Pilar Cordovilla, Maria
通讯作者：
del Pilar Cordovilla, Maria
影响因子：
7.4
作者：
FOYER, C;LELANDAIS, M;KUNERT, KJ
通讯作者：
KUNERT, KJ
影响因子：
5.2
作者：
Gharbi, Emna;Martinez, Juan-Pablo;Lutts, Stanley
通讯作者：
Lutts, Stanley