Molecular epidemiology of human respiratory syncytial virus and human metapneumovirus in hospitalized children with acute respiratory infections in Croatia, 2014-2017
Molecular epidemiology of human respiratory syncytial virus and human metapneumovirus in hospitalized children with acute respiratory infections in Croatia, 2014-2017
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DOI：
10.1016/j.meegid.2019.104039
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发表时间：
2019-12-01
影响因子：
3.2
通讯作者：
Forcic, D.
中科院分区：
文献类型：
--
作者：
Jagusic, M.;Slovic, A.;Forcic, D.
急性呼吸道感染（ARI）是5岁以下儿童最常见的感染，通常由两种肺炎病毒引起，即人呼吸道合胞病毒（HRSV）和人偏肺病毒（HMPV）。这些病毒的流行季节重叠，疾病表现高度相似，包括严重的低ARI，如细支气管炎或肺炎。肺炎病毒的再感染很常见，可获得的预防治疗有限。在连续四年（2014-2017年）期间监测了克罗地亚流行的HRSV和HMPV毒株的遗传多样性。两种病毒均观察到多个谱系的共循环。在HRSV A组，ON 1株获得了很强的优势，在4年期间，而以前的优势基因型NA 1只是零星检测。同样，在此期间，新出现的HMPV基因型A2 c比基因型A2 b占优势，导致2017年的所有感染均由A2 c引起。沿着基于这些病毒的检测和基因分型的常用片段的系统发育分析，还分析了全长G和SH基因。进化动力学表明，HRSV和HMPV的替代率在2.51 × 10（-3）~ 3.61 × 10（-3）个替代/位点/年之间。这项研究确定了最近描述的HMPV毒株在克罗地亚G基因中含有大的重复。具有两个重复中的任何一个的病毒属于子集群A2 c，该子集群在2017年完全取代了所有其他A组子集群。
Acute respiratory infection (ARI) is the most common infection in children under 5 years of age and it is frequently caused by two pneumoviruses, human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). Epidemic seasons of these viruses overlap and disease manifestations are highly similar, including severe lower ARI such as bronchiolitis or pneumonia. Reinfections with pneumoviruses are frequent and limited prevention treatment is available. Genetic diversity of HRSV and HMPV strains circulating in Croatia was monitored during four consecutive years (2014-2017). Co-circulation of multiple lineages was observed for both viruses. Within HRSV group A, ON1 strains gained strong predominance during the 4-year period, while previously dominant genotype NA1 was detected only sporadically. Similarly, newly occurring HMPV genotype A2c gained predominance over genotype A2b during this period, resulting in all infection in 2017 being caused by A2c. Along with phylogenetic analysis based on the commonly used fragments for detection and genotyping of these viruses, full length G and SH genes were also analysed. Evolutionary dynamics showed that inferred substitution rates of HRSV and HMPV are between 2.51 x 10(-3) and 3.61 x 10(-3) substitutions/site/year. This study established presence of recently described HMPV strains containing large duplications in the G gene in Croatia. Viruses with either of the two duplications belong to a subcluster A2c, which has completely replaced all other group A subclusters in 2017.