Efficient Inhibition of Human Influenza A Virus by Oligonucleotides Electrostatically Fixed on Polylysine-Containing TiO2 Nanoparticles
Efficient Inhibition of Human Influenza A Virus by Oligonucleotides Electrostatically Fixed on Polylysine-Containing TiO2 Nanoparticles
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DOI：
10.1134/s1068162014020095
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发表时间：
2014-03-01
影响因子：
1
通讯作者：
Zarytova, V. F.
中科院分区：
文献类型：
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作者：
Levina, A. S.;Repkova, M. N.;Zarytova, V. F.
研究了TiO2中心点PL中心点DNA纳米复合材料对甲型流感病毒（H3N2亚型）感染MDCK细胞的抗病毒活性。纳米复合材料中的DNA片段被静电结合到用聚赖氨酸预先覆盖的二氧化钛纳米颗粒上。结果表明，在低浓度（0.1 μ M和0.2 μ M） DNA（v3′）纳米复合材料中，含有靶向病毒RNA第5段非编码区3′端的DNA（v3′）片段的TiO2中心点PL中心点DNA（v3′）纳米复合材料特异性抑制病毒繁殖的效率分别为99.8%和99.9%（分别为400和1000倍）。含有与病毒RNA不互补的寡核苷酸或未结合的寡核苷酸DNA（v3′）的TiO2中心点PL中心点DNA(r)纳米复合材料显示出非常低的抗病毒活性（抑制因子分别为3.5和1.3）。
Antiviral activity of the TiO2 center dot PL center dot DNA nanobiocomposites was studied on the MDCK cell culture infected with influenza A virus (subtype H3N2). DNA fragments in the nanocomposites are electrostatically bound to titanium dioxide nanoparticles precovered with polylysine. It was shown that TiO2 center dot PL center dot DNA(v3') nanocomposite bearing the DNA(v3') fragment targeted to the 3'-end of the noncoding region of segment 5 of viral RNA specifically inhibited the virus reproduction with the efficiency of 99.8% and 99.9% (i.e., by factors of similar to 400 and 1000, respectively) at a low concentration of DNA(v3') in nanocomposite (0.1 and 0.2 mu M, respectively). The TiO2 center dot PL center dot DNA(r) nanocomposite containing an oligonucleotide noncomplementary to viral RNA or oligonucleotide DNA(v3') unbound to the nanoparticles show very low antiviral activity (inhibition by factors of similar to 3.5 and 1.3, respectively).