Genomic analysis identifies association of Fusobacterium with colorectal carcinoma
Genomic analysis identifies association of Fusobacterium with colorectal carcinoma
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DOI：
10.1101/gr.126573.111
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发表时间：
2012-02-01
期刊：
影响因子：
7
通讯作者：
Meyerson, Matthew
中科院分区：
文献类型：
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作者：
Kostic, Aleksandar D.;Gevers, Dirk;Meyerson, Matthew
结直肠癌的肿瘤微环境是由基因突变的癌细胞、非肿瘤细胞和多种微生物组成的复杂群落。这些成分中的每一种都可能有助于癌症的发生；然而，对微生物区系的作用了解最少。我们使用了九个肿瘤/正常对的全基因组序列来表征结直肠癌微生物区系的组成。对95对癌/正常DNA进行定量聚合酶链式反应和16S rDNA序列分析，证实梭杆菌在癌组织中富含DNA序列，而在肿瘤组织中缺失类杆菌和假单胞菌。FISH还显示了大肠肿瘤内的梭杆菌。这些发现揭示了结直肠癌微生物区系的变化；然而，梭菌在结直肠癌发病机制中的确切作用还需要进一步研究。
The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.