Mobilisation of endothelial progenitor cells: one of the possible mechanisms involved in the chronic administration of melatonin preventing erectile dysfunction in diabetic rats
Mobilisation of endothelial progenitor cells: one of the possible mechanisms involved in the chronic administration of melatonin preventing erectile dysfunction in diabetic rats
复制标题
内皮祖细胞的动员：长期服用褪黑激素预防糖尿病大鼠勃起功能障碍的可能机制之一。
DOI：
10.1038/aja.2011.161
复制
发表时间：
2012-05-01
影响因子：
2.9
通讯作者：
Dai, Yu-Tian
中科院分区：
文献类型：
--
作者：
Qiu, Xue-Feng;Li, Xiao-Xin;Dai, Yu-Tian
糖尿病引起的氧化应激在内皮祖细胞(EPC)从骨髓到循环的动员中起着关键作用。本研究旨在探讨慢性应用褪黑素对I型糖尿病大鼠内皮祖细胞动员和勃起功能的影响。对链脲佐菌素诱导的I型糖尿病大鼠给予褪黑素。用流式细胞仪检测EPC水平。用超氧化物歧化酶和丙二醛的水平来表示骨髓中的氧化应激。通过测量电刺激海绵体神经时海绵体内压力来评估勃起功能。用免疫组织化学方法检测海绵体内皮细胞密度、平滑肌和胶原的比例。褪黑素可提高小鼠骨髓超氧化物歧化酶水平，降低丙二醛水平。这一效应伴随着糖尿病大鼠循环内皮祖细胞水平的增加。治疗组大鼠海绵体内压/平均动脉压比明显高于糖尿病对照组大鼠。组织学分析显示，在给予褪黑素后，海绵体的内皮密度增加。然而，褪黑素治疗并未改变糖尿病大鼠海绵体中平滑肌和胶原的比例。长期服用褪黑素对预防I型糖尿病大鼠勃起功能障碍(ED)有良好效果。促进内皮祖细胞的动员是改善ED的可能机制之一。
Diabetes-induced oxidative stress plays a critical role in the mobilisation of endothelial progenitor cells (EPCs) from the bone marrow to the circulation. This study was designed to explore the effects of chronic melatonin administration on the promotion of the mobilisation of EPCs and on the preservation of erectile function in type I diabetic rats. Melatonin was administered to streptozotocin-induced type I diabetic rats. EPCs levels were determined using flow cytometry. Oxidative stress in the bone marrow was indicated by the levels of superoxide dismutase and malondialdehyde. Erectile function was evaluated by measuring the intracavernous pressure during an electrostimulation of the cavernous nerve. The density of the endothelium and the proportions of smooth muscle and collagen in the corpus cavernosum were determined by immunohistochemistry. The administration of melatonin increased the superoxide dismutase level and decreased the malondialdehyde level in the bone marrow. This effect was accompanied by an increased level of circulating EPCs in the diabetic rats. The intracavernous pressure to mean arterial pressure ratio of the rats in the treatment group was significantly greater, compared with diabetic control rats. The histological analysis demonstrated an increase in the endothelial density of the corpus cavernosum after the administration of melatonin. However, melatonin treatment did not change the proportions of smooth muscle and collagen in the corpus cavernosum of diabetic rats. Chronic administration of melatonin has a beneficial effect on preventing erectile dysfunction (ED) in type I diabetic rats. Promoting the mobilisation of EPCs is one of the possible mechanisms involved in the improvement of ED.