Inhibition of Migration and Invasion by Tet-1 Overexpression in Human Lung Carcinoma H460 Cells.
Inhibition of Migration and Invasion by Tet-1 Overexpression in Human Lung Carcinoma H460 Cells.
复制标题
DOI：
10.3727/096504015x14496932933539
复制
发表时间：
2016
影响因子：
3.1
通讯作者：
Ryoo ZY
中科院分区：
文献类型：
--
作者：
Park SJ;Lee BR;Kim HS;Ji YR;Sung YH;ShikChoi K;Park HD;Kim SH;Kim MO;Ryoo ZY
在本研究中，我们发现表达Tet1的肺癌细胞系（H460细胞）表现出更高水平的粘附，并且Tet1抑制H460细胞增殖。此外，与对照组相比，这些细胞的胶原蛋白降解和 Smad2/3 磷酸化能力显着降低。此外，波形蛋白被发现在较大的转移性癌症区域中高表达。上皮标记物 E-钙粘蛋白中 Tet1 过度表达减少。此外，Tet1 还可抑制裸鼠体内的癌细胞转移。总的来说，这些发现表明 Tet1 表达通过抑制侵袭和上皮间质转化 (EMT) 在肺癌细胞的转移中发挥着关键作用。
In the present study, we found that lung cancer cell line (H460 cells) expressing Tet1 showed higher levels of adhesion, and Tet1 inhibited H460 cell proliferation. In addition, these cells showed a significantly reduced ability of collagen degradation and Smad2/3 phosphorylation compared to controls. Furthermore, vimentin was found to be highly expressed in larger metastatic cancer area. Tet1 overexpression was reduced in the epithelial marker E-cadherin. Moreover, Tet1 repressed cancer cell metastasis in nude mice. Collectively, these findings suggest that Tet1 expression plays a critical role in metastasis of lung cancer cells by suppression of invasion and epithelial–mesenchymal transition (EMT).