Ascorbate peroxidase 1 confers resistance to southern corn leaf blight in maize
Ascorbate peroxidase 1 confers resistance to southern corn leaf blight in maize
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抗坏血酸过氧化物酶 1 赋予玉米抗南方玉米叶枯病的能力
DOI：
10.1111/jipb.13254
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发表时间：
2022
影响因子：
11.4
通讯作者：
Wu Liuji
中科院分区：
文献类型：
--
作者：
Zhang Jinghua;Jia Xingmeng;Wang Guanfeng;Ma Shijun;Wang Shunxi;Yang Qin;Chen Xueyan;Zhang Yuqian;Lyu Yajing;Wang Xiaoxu;Shi Jiawei;Zhao Yangtao;Chen Yanhui;Wu Liuji
玉米大斑病（Southern corn leaf blight，SCLB）是由玉米小斑病（Bipolaris maydis）引起的玉米大斑病（Southern corn leaf blight，SCLB），是影响玉米生产的主要病害之一。我们进行了比较蛋白质组学分析，鉴定了258种蛋白质，这些蛋白质在玉米对玉米B.maydis的反应过程中表现出不同的丰度。抗坏血酸过氧化物酶玉米抗坏血酸过氧化物酶1（ZmAPX 1）基因位于一个与SCLB抗性相关的数量性状基因的定位区间内，ZmAPX 1的过表达导致H_2O_2积累减少，对玉米B.maydis的抗性增强。过表达被玉米B.maydis感染的植物，而Zmapx 1突变体则表现出相反的作用。我们进一步确定低水平的H_2O_2伴随着JA的积累，从而增强SCLB抗性。这些结果表明ZmAPX 1通过减少H_2O_2的积累和激活JA-来正向调节SCLB抗性。ZmAPX 1是一个潜在的提高SCLB抗性的基因，为进一步阐明植物APXs的功能提供了依据。
Southern corn leaf blight (SCLB),caused by Bipolaris maydis,is one of the most devastating diseases affecting maize production.However,only one SLCB resistance gene,conferring partial resistance,is currently known,underscoring the importance of isolating new SCLB resistance-related genes.Here,we performed a comparative proteomic analysis and identified 258 proteins showing differential abundance during the maize response to B.maydis.These proteins included an ascorbate peroxidase (Zea mays ascorbate peroxidase 1 (ZmAPX1)) encoded by a gene located within the mapping interval of a previously identified quantitative trait locus associated with SCLB resistance.ZmAPX1 overexpression resulted in lower H_2O_2 accumulation and enhanced resistance against B.maydis.Jasmonic acid (JA) contents and transcript levels for JA biosynthesis and responsive genes increased in ZmAPXI-overexpressing plants infected with B.maydis,whereas Zmapx1 mutants showed the opposite effects.We further determined that low levels of H_2O_2 are accompanied by an accumulation of JA that enhances SCLB resistance.These results demonstrate that ZmAPX1 positively regulates SCLB resistance by decreasing H_2O_2 accumulation and activating the JA-mediated defense signaling pathway.This study identified ZmAPX1 as a potentially useful gene for increasing SCLB resistance.Furthermore,the generated data may be relevant for clarifying the functions of plant APXs.