Basic fibroblast growth factor eluting microspheres enhance distraction enterogenesis
Basic fibroblast growth factor eluting microspheres enhance distraction enterogenesis
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DOI：
10.1016/j.jpedsurg.2016.02.065
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发表时间：
2016-06-01
影响因子：
2.4
通讯作者：
Dunn, James C. Y.
中科院分区：
文献类型：
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作者：
Rouch, Joshua D.;Scott, Andrew;Dunn, James C. Y.
目的：本研究的目的是确定使用自膨胀聚己内酯（PCL）弹簧的牵拉肠发生是否是短肠综合征的潜在治疗方法。缓释碱性成纤维细胞生长因子（bFGF）微球在组织工程支架中具有诱导血管生成和肠道再生的作用。我们假设提供负载bfgf的微球会增加血管生成，从而促进肠形成过程。方法：取啮齿类动物空肠10 mm段，置入包封的PCL弹簧。空白或bfgf负载的微球被递送到片段。4周后，评估空肠节段的长度、形态、血管和神经节的量化。-结果：与空白微球相比，接受bFGF微球的延长肠段微血管密度显著增加。在接受bFGF微球治疗的节段中，粘膜下神经节和myenteric神经节也明显增多。空白组和bFGF组的空肠节段长度和最终肌厚相似，但bFGF微球导致空肠节段管腔直径显著增加。结论：缓释bFGF微球通过改善血管功能促进牵张肠发生。生长因子如bFGF与牵张性肠发生的协同作用可能会改善短肠综合征患者未来的治疗效果。(C) 2016 Elsevier Inc.版权所有。
Purpose: The purpose of this study was to determine if distraction enterogenesis using self-expanding polycaprolactone (PCL) springs is a potential therapy for short bowel syndrome. Sustained release basic fibroblast growth factor (bFGF) microspheres have been shown to induce angiogenesis and intestinal regeneration in tissue engineered scaffolds. We hypothesized that the provision of bFGF-loadedmicrospheres would increase angiogenesis and thereby enhance the process of enterogenesis.Methods: A 10-mm segment of rodent jejunum was isolated and an encapsulated PCL spring inserted. Blank or bFGF-loaded microspheres were delivered to the segment. After 4 weeks, jejunal segments were assessed for lengthening, morphology, quantification of blood vessels, and ganglia.-Results: Lengthened intestinal segments receiving bFGF microspheres demonstrated significantly increased microvascular density compared to those with blank microspheres. There were also significantly more submucosal andmyenteric ganglia in the segments that received bFGF microspheres. Segments achieved similar lengthening and final muscular thickness in both blank and bFGF groups, but the bFGF microsphere caused a significant increase in luminal diameter of the jejunal segment.Conclusion: Sustained release bFGF microspheres enhanced distraction enterogenesis through improved vascularity. The synergy of growth factors such as bFGF with distraction enterogenesis may yield improved results for the future treatment of patients with short bowel syndrome. (C) 2016 Elsevier Inc. All rights reserved.