Cerebrospinal fluid to brain transport of manganese in a non-human primate revealed by MRI
Cerebrospinal fluid to brain transport of manganese in a non-human primate revealed by MRI
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DOI：
10.1016/j.brainres.2007.12.065
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发表时间：
2008-03-10
期刊：
影响因子：
2.9
通讯作者：
Silva, Afonso C.
中科院分区：
文献类型：
--
作者：
Bock, Nicholas A.;Paiva, Fernando F.;Silva, Afonso C.
在非人类灵长类动物和人类中过量接触锰会导致一种叫做锰中毒的神经退行性疾病，这种疾病被认为与金属在基底神经节中的积累有关。在这里，我们利用t -1加权磁共振成像（MRI）评估了非人类灵长类动物（普通狨猴，Callithrix jacchus）在尾静脉全身注射30 mg/kg mncl2h2o四次后大脑区域锰浓度的变化，并将这些变化与相同暴露途径和剂量的大鼠进行了比较。每次给药间隔48小时，最后一次给药后48小时对动物进行成像。我们发现，与大鼠相比，狨猴在大脑区域有明显更大的t -1加权图像增强，特别是在基底神经节和视觉皮层。为了证实这种物种间的差异反映了锰浓度的实际差异，而不是锰的MRI性质的变化，我们测量了体内大脑中锰的纵向弛豫度（chi(1)），没有发现明显的物种差异。狨猴基底节区和视觉皮层的高锰摄取可以通过大侧脑室的csf脑运输来解释，我们在尾静脉注射锰时通过时间过程MRI证实了这一摄取途径。在靠近脑室的海马体亚结构中也有高摄取。过度暴露在这些结构中的大量锰积累可能对包括人类在内的所有灵长类动物都是常见的。Elsevier B.V.出版
Manganese overexposure in non-human primates and humans causes a neurodegenerative disorder called manganism thought to be related to an accumulation of the metal in the basal ganglia. Here, we assess changes in the concentration of manganese in regions of the brain of a non-human primate (the common marmoset, Callithrix jacchus) following four systemic injections of 30 mg/kg MnCl2 H2O in the tail vein using T-1-weighted magnetic resonance imaging (MRI) and compare these to changes in the rat following the same exposure route and dose. The doses were spaced 48 h apart and we imaged the animals 48 h after the final dose. We find that marmosets have significantly larger T-1-weighted image enhancements in regions of the brain compared to rats, notably in the basal ganglia and the visual cortex. To confirm this difference across species reflects actual differences in manganese concentrations and not variations in the MRI properties of manganese, we measured the longitudinal relaxivity of manganese (chi(1)) in the in vivo brain and found no significant species' difference. The high manganese uptake in the marmoset basal ganglia and visual cortex can be explained by CSF-brain transport from the large lateral ventricles and we confirm this route of uptake with time-course MRI during a tail-vein infusion of manganese. There is also high uptake in the substructures of the hippocampus that are adjacent to the ventricles. The large manganese accumulation in these structures on overexposure may be common to all primates, including humans. Published by Elsevier B.V.