Primary and secondary structure of bovine retinal S antigen (48-kDa protein).
Primary and secondary structure of bovine retinal S antigen (48-kDa protein).
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牛视网膜 S 抗原（48-kDa 蛋白）的一级和二级结构。
DOI：
10.1073/pnas.84.20.6975
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发表时间：
1987
影响因子：
11.1
通讯作者：
Tao,R
中科院分区：
文献类型：
--
作者：
Shinohara,T;Dietzschold,B;Craft,CM;Wistow,G;Early,JJ;Donoso,LA;Horwitz,J;Tao,R
用核酸序列分析和部分氨基酸序列测定了牛S抗原(48 kDa蛋白)的氨基酸全序列。该基因全长1623个碱基对，含有一个开放阅读框，编码404个氨基酸(45275Da)的蛋白质。纯化了天然牛S抗原的胰多肽和溴化氰多肽，并对其进行了部分序列测定。所有这些多肽都出现在长的开放阅读框中。检索国家生物医学研究基金会数据库，发现S抗原与其他蛋白质没有广泛的序列同源性。然而，存在与α转导蛋白序列相似的局部区域，包括受百日咳杆菌和霍乱毒素的ADP核糖基化作用的部位和磷酸化结合部位。二级结构预测和圆二色谱分析表明，S抗原主要由β-折叠构象组成。酸催化的甲烷分解反应表明分子中存在低水平的碳水化合物。
The complete amino acid sequence of bovine S antigen (48-kDa protein) has been determined by cDNA and partial amino acid sequencing. A 1623-base-pair (bp) cDNA contains an open reading frame coding for a protein of 404 amino acids (45,275 Da). Tryptic peptides and cyanogen bromide peptides of native bovine S antigen were purified and partially sequenced. All of these peptides were accounted for in the long open reading frame. Searching of the National Biomedical Research Foundation data bank revealed no extensive sequence homology between S antigen and other proteins. However, there are local regions of sequence similarity with alpha transducin, including the sites subject to ADP-ribosylation by Bordetella pertussis and cholera toxins and the phosphoryl binding-sites. Secondary structure prediction and circular dichroic spectroscopy show that S antigen is composed predominantly of beta-sheet conformation. Acid-catalyzed methanolysis suggests the presence of low levels of carbohydrate in the molecule.