Evaluation of commercial selective agars in screening for methicillin-resistant Staphylococcus aureus.
Evaluation of commercial selective agars in screening for methicillin-resistant Staphylococcus aureus.
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商业选择性琼脂筛选耐甲氧西林金黄色葡萄球菌的评估。
DOI：
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发表时间：
2010
影响因子：
0.8
通讯作者：
Hee
中科院分区：
文献类型：
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作者：
Hee;J. Suh;Hee
目的评价耐甲氧西林沙门氏菌的耐药性。金黄色葡萄球菌（MRSA）检测方法，我们比较了（a）甘露醇盐琼脂与头孢西丁（MSA-FX），（B）MRSA选择琼脂（Bio-Rad），（c）MRSA ID（bioMerieuex），和（d）CHROMagar MRSA（BD Diagnostics）作为用于培养从重症监护病房（ICU）患者和医护人员收集的鼻拭子样本的选择性培养基。从578份标本中共回收99例（17.1%）MRSA。4例（5.5%）病例来自医护人员，95例（18.8%）来自ICU患者。18小时后，MSA-FX、MRSA Select、MRSA ID和CHROMagar MRSA的灵敏度分别为83.8、87.9、80.8和84.8%; 24小时后分别为92.9、94.9、90.9和91.9%; 48小时后分别为96.0、100、99.0和99.0%。MSA-FX、MRSA Select、MRSA ID和CHROMagar MRSA的特异性分别为97.7、99.0和98.7。18小时后分别为99.8%; 24小时后分别为97.1、98.5、98.1和99.5%; 48小时后分别为95.2、97.7、97.9和99.0%。总之，所有四种培养基在24小时读数后均显示出良好的结果，但MRSA ID和CHROMagar MRSA培养基需要在48小时读数，因为该时间点的灵敏度增加。
To evaluate methicillin-resistant S. aureus (MRSA) detection methods, we compared (a) mannitol salt agar with cefoxitin (MSA-FX), (b) MRSASelect agar (Bio-Rad), (c) MRSA ID (bioMerieuex), and (d) CHROMagar MRSA (BD Diagnostics) as selective media for culturing nasal swab specimens collected from intensive care unit (ICU) patients and healthcare personnel. A total of 99 (17.1%) cases of MRSA were recovered from 578 specimens. Four (5.5%) cases were identified from healthcare personnel and 95 (18.8%) were from ICU patients. The sensitivity of MSA-FX, MRSASelect, MRSA ID, and CHROMagar MRSA was 83.8, 87.9, 80.8, and 84.8% after 18 hr; 92.9, 94.9, 90.9, and 91.9% after 24 hr; and 96.0, 100, 99.0, and 99.0% after 48 hr, respectively. The specificity of MSA-FX, MRSASelect, MRSA ID, and CHROMagar MRSA was 97.7, 99.0, 98.7. and 99.8% after 18 hr; 97.1, 98.5, 98.1, and 99.5% after 24 hr; and 95.2, 97.7, 97.9, and 99.0% after 48 hr, respectively. In conclusion, all four media showed good results after the 24 hr readings, but MRSA ID and CHROMagar MRSA media required readings at 48 hr due to increased sensitivity at this time point.