PI3K/AKT/mTOR signaling pathway inhibitors in proliferation of retinal pigment epithelial cells
PI3K/AKT/mTOR signaling pathway inhibitors in proliferation of retinal pigment epithelial cells
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DOI：
10.3980/j.issn.2222-3959.2012.06.05
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发表时间：
2012-12-18
影响因子：
1.4
通讯作者：
Chen, Lei
中科院分区：
文献类型：
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作者：
Cai, Na;Dai, Shun-Dong;Chen, Lei
目的：确定在人类增殖性玻璃体视网膜病变（PVR）中PI3K/AKT/mTOR通路是否被激活。
方法：收集对照组和PVR患者的视网膜，通过蛋白质印迹法测定PI3K、磷酸化AKT、磷酸化mTOR、磷酸化p70S6k和磷酸化4EBP - 1的水平。用不同浓度和作用时间的特异性mTOR抑制剂雷帕霉素（RAPA）或PI3K抑制剂LY294002处理培养的人视网膜色素上皮细胞系D407。通过相差显微镜观察细胞形态，通过MTT法和流式细胞术测定处理细胞的增殖和凋亡情况。
结果：PVR患者视网膜中PI3K、磷酸化AKT、磷酸化mTOR、磷酸化P70S6K和磷酸化4EBP1的水平升高（P
AIM: To determine whether the PI3K/AKT/mTOR pathway is activated in proliferative vitreoretinopathy (PVR) in homo-sapiens.METHODS: The retina of controls and patients with PVR were collected and their levels of PI3K, phospho-AKT, phospho-mTOR, phospho-p70S6k and phospho-4EBP-1 were determined by Western blot. The cultured human retinal pigment epithelial cell line D407 was treated with a specific mTOR inhibitor, rapamycin (RAPA) or a PI3K inhibitor, LY294002, of various concentrations and durations. Cell morphology was observed by phase contrast microscopy and the proliferation and apoptosis of treated cells were determined by MTT assay and flow cytometry.RESULTS: Levels of PI3K, phospho-AKT, phospho-mTOR, phospho-P70S6K and phospho-4EBP1 was increased in the retina in PVR (P