Heavy metal-induced glutathione accumulation and its role in heavy metal detoxification in Phanerochaete chrysosporium
Heavy metal-induced glutathione accumulation and its role in heavy metal detoxification in Phanerochaete chrysosporium
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重金属诱导的黄孢原毛平革菌谷胱甘肽积累及其在重金属解毒中的作用
DOI：
10.1007/s00253-014-5667-x
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
He Yibin
中科院分区：
文献类型：
--
作者：
Lai Cui;Zhao Meihua;Huang Chao;Li Ningjie;Wei Zhen;Wu Haipeng;Zhang Chen;Lai Mingyong;He Yibin
黄孢原毛平革菌是一种重要的重金属超富集菌，具有良好的细胞内富集能力。本研究分析了重金属诱导的黄孢原毛平革菌谷胱甘肽（GSH）积累及其在细胞内重金属水平的调控。黄孢原毛平革菌积累了高水平的谷胱甘肽，同时细胞内铅和镉浓度也很高。铅的生物积累导致GSH积累波动范围很窄（0.72-0.84 μmol），而镉的生物积累导致GSH积累急剧下降，最大值为0.37 μmol。GSH的动态变化与Cd的生物累积之间存在良好的相关性（R（2）> 0.87），而与Pb的生物累积之间没有显著的相关性（R（2）< 0.38）。Pb/GSH的摩尔比在0.10 ~ 0.18之间，Cd/GSH的摩尔比在1.53-3.32之间，表明GSH在Cd螯合中起主导作用。研究还表明，黄孢原毛平革菌对Pb离子表现出了相当的超耐受性，并伴随着GSH合成的需求驱动刺激和不明显的活性氧胁迫的产生。GSH对Cd的亲和力较强，主要作为Cd的螯合剂参与Cd的解毒机制，从而导致GSH对Cd的反应急剧下降。GSH代谢及其在改善金属毒性中的作用的研究可以为微生物在废水处理中的应用提供重要信息。
Phanerochaete chrysosporium are known to be vital hyperaccumulation species for heavy metal removal with admirable intracellular bioaccumulation capacity. This study analyzes the heavy metal-induced glutathione (GSH) accumulation and the regulation at the intracellular heavy metal level in P. chrysosporium. P. chrysosporium accumulated high levels of GSH, accompanied with high intracellular concentrations of Pb and Cd. Pb bioaccumulation lead to a narrow range of fluctuation in GSH accumulation (0.72-0.84 μmol), while GSH plummeted under Cd exposure at the maximum value of 0.37 μmol. Good correlations between time-course GSH depletion and Cd bioaccumulation were determined (R (2) > 0.87), while no significant correlations have been found between GSH variation and Pb bioaccumulation (R (2) < 0.38). Significantly, concentration-dependent molar ratios of Pb/GSH ranging from 0.10 to 0.18 were observed, while molar ratios of Cd/GSH were at the scope of 1.53-3.32, confirming the dominant role of GSH in Cd chelation. The study also demonstrated that P. chrysosporium showed considerable hypertolerance to Pb ions, accompanied with demand-driven stimulation in GSH synthesis and unconspicuous generation of reactive oxygen stress. GSH plummeted dramatically response to Cd exposure, due to the strong affinity of GSH to Cd and the involvement of GSH in Cd detoxification mechanism mainly as Cd chelators. Investigations into GSH metabolism and its role in ameliorating metal toxicity can offer important information on the application of the microorganism for wastewater treatment.
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影响因子：
7.4
作者：
Cobbett, CS
通讯作者：
Cobbett, CS
影响因子：
11.4
作者：
G. Zeng;Danlian Huang;G. Huang;Tianjue Hu;Xiao-yun Jiang;C. Feng;Yaoning Chen;L. Tang;Hong-liang Liu
通讯作者：
G. Zeng;Danlian Huang;G. Huang;Tianjue Hu;Xiao-yun Jiang;C. Feng;Yaoning Chen;L. Tang;Hong-liang Liu
DOI：
--
发表时间：
2008-10
期刊：
The Indian journal of medical research
影响因子：
--
作者：
S. Flora;Megha Mittal;A. Mehta
通讯作者：
S. Flora;Megha Mittal;A. Mehta
影响因子：
3.8
作者：
M. Tsezos;B. Volesky
通讯作者：
M. Tsezos;B. Volesky
影响因子：
11.4
作者：
A. Falih
通讯作者：
A. Falih