Sensory mechanisms controlling the timing of larval developmental and behavioral transitions require the Drosophila DEG/ENaC subunit, Pickpocket1
Sensory mechanisms controlling the timing of larval developmental and behavioral transitions require the Drosophila DEG/ENaC subunit, Pickpocket1
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DOI：
10.1016/j.ydbio.2008.07.003
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发表时间：
2008-10-01
影响因子：
2.7
通讯作者：
Johnson, Wayne A.
中科院分区：
文献类型：
--
作者：
Ainsley, Joshua A.;Kim, Myung Jun;Johnson, Wayne A.
多细胞生物的生长通过一系列精确定时的发育事件进行，需要基因表达、行为变化和环境条件之间的协调。在果蝇幼虫中，从觅食（摄食）到徘徊（非摄食）行为的重要中三龄转变发生在化蛹和变态之前。这一关键转变的时间与幼虫的生长和大小相协调。但人们对调节这一过程的生理机制知之甚少。这里提出的结果表明，果蝇幼虫将特定的环境条件（例如温度）与食物联系起来，以便制定适当的觅食策略。从觅食行为到徘徊行为的转变与对食物相关刺激的行为反应的显着逆转有关，这种逆转始于第三龄早期，远在食物退出之前。破坏 Degenerin/上皮钠通道亚基、扒手 I (PPK1) 表达或 PPK1 外周感觉神经元功能的基因操作会导致这些行为转变的时间缺陷。瞬时失活实验表明，在第三龄早期的关键时期需要来自 PPK1 神经元的感觉输入来影响这一发育转变。结果表明，PPK1 感觉神经元在调节与幼虫从觅食到游走阶段的发育进程相关的重要行为转变中发挥着关键作用。 (c) 2008 Elsevier Inc. 保留所有权利。
Growth of multicellular organisms proceeds through a series of precisely timed developmental events requiring Coordination between gene expression, behavioral changes, and environmental conditions. In Drosophila melanogaster larvae, the essential midthird instar transition from foraging (feeding) to wandering (non-feeding) behavior occurs prior to pupariation and metamorphosis. The timing of this key transition is coordinated with larval growth and size. but physiological mechanisms regulating this process are poorly understood. Results presented here show that Drosophila larvae associate specific environmental conditions, such as temperature, with food in order to enact appropriate foraging strategies. The transition from foraging to wandering behavior is associated with a striking reversal in the behavioral responses to food-associated stimuli that begins early in the third instar, well before food exit. Genetic manipulations disrupting expression of the Degenerin/Epithelial Sodium Channel subunit, Pickpocket I (PPK1) or function of PPK1 peripheral sensory neurons caused defects in the timing of these behavioral transitions. Transient inactivation experiments demonstrated that sensory input from PPK1 neurons is required during a critical period early in the third instar to influence this developmental transition. Results demonstrate a key role for the PPK1 sensory neurons in regulation of important behavioral transitions associated with developmental progression of larvae from foraging to wandering stage. (c) 2008 Elsevier Inc. All rights reserved.