Highly efficient electrochemical sensing platform for sensitive detection DNA methylation, and methyltransferase activity based on Ag NPs decorated carbon nanocubes
Highly efficient electrochemical sensing platform for sensitive detection DNA methylation, and methyltransferase activity based on Ag NPs decorated carbon nanocubes
复制标题
基于Ag NPs修饰碳纳米立方体的高效电化学传感平台，用于灵敏检测DNA甲基化和甲基转移酶活性
DOI：
10.1016/j.bios.2017.07.063
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发表时间：
2018-01-15
影响因子：
12.6
通讯作者：
Geng, Deqin
中科院分区：
文献类型：
--
作者：
Gao, Fenglei;Fan, Taotao;Geng, Deqin
本文报道了一种以银纳米粒子修饰的碳纳米立方体（CNCs）为信号标记物的电化学方法，用于DNA甲基化的定量、MTase活性的分析和MTase抑制剂的筛选。通过在羧基化的CNCs上原位生长纳米银来制备Ag NPs/CNCs，并将其用作示踪标签来标记抗体。该传感器是将DNA双螺旋结构固定在金电极表面制成的。当引入DNA MTase时，探针被甲基化。随后，抗5-甲基胞嘧啶抗体标记的Ag NPs/CNCs特异性地缀合在CpG甲基化位点上。银纳米颗粒的电化学溶出信号用于监测MTase的活性。电化学信号与M.SssI的活性在0.05 ~ 120 U/mL范围内呈线性关系，检出限为0.03 U/mL。该方法可用于MTase抑制剂的快速评价和筛选。新设计的策略避免了电化学分析的脱氧要求，因此提供了有希望的临床应用潜力。
In this paper, we reported a sensitive and selective electrochemical method for quantify DNA methylation, analyzing DNA MTase activity and screening of MTase inhibitor based on silver nanoparticles (Ag NPs) decorated carbon nanocubes (CNCs) as signal tag. The Ag NPs/CNCs was prepared by in situ growth of nanosilver on carboxylated CNCs and used as a tracing tag to label antibody. The sensor was prepared by immobilizing the double DNA helix structure on the surface of gold electrode. When DNA MTase was introduced, the probe was methylated. Successively, anti-5-methylcytosine antibody labeled Ag NPs/CNCs was specifically conjugated on the CpG methylation site. The electrochemical stripping signal of the Ag NPs was used to monitor the activity of MTase. The electrochemical signal has a linear relationship with M.SssI activities ranging from 0.05 to 120 U/mL with a detection limit of 0.03 U/mL. In addition, we also demonstrated the method could be used for rapid evaluation and screening of the inhibitors of MTase. The newly designed strategy avoid the requirement of deoxygenation for electrochemical assay, and thus provide a promising potential in clinical application.