The functional and structural response to distention of the rabbit whole bladder in vitro
The functional and structural response to distention of the rabbit whole bladder in vitro
复制标题
DOI：
10.1016/s0022-5347(05)64243-7
复制
发表时间：
2002-12-01
影响因子：
6.6
通讯作者：
Levin, RM
中科院分区：
文献类型：
--
作者：
Matsumoto, S;Chichester, P;Levin, RM
目的：我们研究了扩张对膀胱内压力产生的影响，以响应刺激的关系，其对膀胱壁厚度和morphology.Materials和方法：共25只新西兰白色兔分为5组，每组5只。每个体外全膀胱制备物均填充生理盐水至5%（未扩张）、25%、50%、100%或125%容量（60 ml）。场刺激，腺苷5 '-三磷酸，氨甲酰胆碱氯化物（卡巴胆碱）和氯化钾对膀胱内压力产生的影响进行了测定。额外的膀胱同样膨胀（3在每一个容量的百分比），固定在福尔马林和评估semiquantitative morphometry.Results：在响应现场刺激的压力产生是最大的5%的容量，其后逐步下降。与未扩张膀胱的反应相比，在25%容量时，对卡巴胆碱的反应增加，然后在25%以上逐渐下降。扩张对KCl的反应没有显著影响。有趣的是，与5%膀胱容量相比，在25%膀胱容量下，响应于腺苷5 '-三磷酸的压力产生增加了4倍以上，并且此后没有显著变化。在25%容量下，膀胱壁厚度减少超过50%，在125%容量下，膀胱壁厚度减少额外12%。最大的变薄，观察粘膜相比，粘膜下层和muscularis.Conclusions：膀胱扩张显着降低了压力的产生，在响应字段刺激，表明扩张降低了有效性的突触传递，而不改变毒蕈碱受体功能或膜去极化。对场刺激的反应的最大变化（减少）发生在5%（未膨胀）和25%容量之间，这是最影响膀胱壁变薄的膨胀变化。
Purpose: We investigated the effects of distention on intravesical pressure generation in response to stimuli in relation to its effects on bladder wall thickness and morphology.Materials and Methods: A total of 25 New Zealand White rabbits were separated into 5 groups of 5 each. Each in vitro whole bladder preparation was filled with saline to 5% (undistended), 25%, 50%, 100% or 125% capacity (60 ml.). The effects of field stimulation, adenosine 5'-triphosphate, carbamylcholine chloride (carbachol) and KCl on intravesical pressure generation were determined. Additional bladders were similarly distended (3 at each percent of capacity), fixed in formalin and evaluated by semiquantitative morphometry.Results: Pressure generation in response to field stimulation was maximal at 5% of capacity and it decreased progressively thereafter. Compared with the responses of undistended bladders, at 25% capacity the response to carbachol increased and then decreased progressively above 25%. Distention had no significant effect on the response to KCl. Interestingly pressure generation in response to adenosine 5'-triphosphate was increased more than 4-fold at 25% bladder capacity compared with 5% and it did not change significantly thereafter. Bladder wall thickness was decreased more than 50% at 25% capacity and by an additional 12% at 125% capacity. The greatest thinning was observed in the mucosa compared with the submucosa and muscularis.Conclusions: Bladder distention significantly reduced pressure generation in response to field stimulation only, indicating that distention reduced the effectiveness of synaptic transmission without altering muscarinic receptor function or membrane depolarization. The greatest change (decrease) in the response to field stimulation occurred between 5% (undistended) and 25% capacity, which was the change in distention that most affected bladder wall thinning.