Energy-dependent activation of spore-lytic enzyme precursor by germinated spores of Clostridium perfringens.
Energy-dependent activation of spore-lytic enzyme precursor by germinated spores of Clostridium perfringens.
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产气荚膜梭菌萌发孢子对孢子裂解酶前体的能量依赖性激活。
DOI：
10.1016/0006-291x(84)90253-5
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
T. Tsuzuki
中科院分区：
文献类型：
--
作者：
Y. Ando;T. Tsuzuki
产气荚膜梭菌的孢子裂解酶的前体是用碱从该生物体的休眠孢子中提取的。通过将酶前体与用碱处理过的萌发孢子一起孵育来活化酶前体。通过添加3-磷酸甘油酸大大增强了激活，表明前体转化为活性酶依赖于萌发过程中的内源能量产生代谢。
A precursor of the spore-lytic enzyme of Clostridium perfringens was extracted with alkali from dormant spores of the organism. The enzyme precursor was activated by incubating it with germinated spores which had been treated with alkali. The activation was greatly enhanced by the addition of 3-phosphoglycerate, suggesting that the conversion of precursor to active enzyme depends on endogenous energy-producing metabolism during germination.