An experimental study of COMP (cartilage oligomeric matrix protein) in the rabbit menisci
An experimental study of COMP (cartilage oligomeric matrix protein) in the rabbit menisci
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DOI：
10.1007/s00402-011-1332-3
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发表时间：
2011-08-01
影响因子：
2.3
通讯作者：
Gomez-Barrena, E.
中科院分区：
文献类型：
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作者：
Lopez-Franco, Mariano;Lopez-Franco, O.;Gomez-Barrena, E.
继发性膝关节骨关节炎（OA）目前与关节损伤有关，但其发病机制尚不清楚。为探讨半月板退变早期软骨细胞和软骨寡聚基质蛋白（COMP）的分布及其变化规律，对10只新西兰兔行右膝前交叉韧带（ACL）切断术。左膝关节作为对照。在4周和12周时处死动物。对半月板和关节软骨的大体损伤进行评分。半月板组织用抗COMP的特异性抗体，Ki-67，使用TUNEL测定和阿辛蓝染色进行免疫染色。ACL切断后4周，2/5的手术膝关节出现关节损伤和内侧韧带撕裂。半月板细胞数量增加，分裂期细胞增多，凋亡细胞增多，COMP和蛋白多糖增多，12周时5/5内侧半月板和2/5外侧半月板出现撕裂，所有手术膝关节软骨均出现骨关节炎性改变。半月板细胞恢复到正常数量，而活跃的细胞分裂低于正常水平，凋亡事件仍然很高，COMP仍然升高，糖胺聚糖甚至更highered.Extracellular基质的变化和改变细胞分布发生在早期的退行性半月板。在继发性OA发病时，关节软骨的变化与关节炎之间存在密切关系。
Secondary knee osteoarthritis (OA) is currently associated with meniscal injuries, but the pathogenesis is unclear. We analyzed the distribution of cells and cartilage oligomeric matrix protein (COMP) and its changes in the early stages of degeneration in meniscus.Ten New Zealand rabbits underwent anterior cruciate ligament (ACL)-transection of the right knee-joint. Left knee-joints were used as controls. The animals were killed at 4 and 12 weeks. Gross injuries in meniscus and articular cartilage were scored. Meniscal tissues were immunostained with a specific antibody against COMP, with Ki-67, using TUNEL-assay and alcian blue stain. The number of cells was counted.At 4 weeks post-ACL-transection, 2/5 of the operated knees showed articular damages and medial menisci tears. Menisci showed a weak increase of cells, higher in cells under division and an increase of apoptosis, COMP and proteoglycans.At 12 weeks, 5/5 of the medial menisci and 2/5 of lateral menisci presented tears, and osteoarthritic changes were seen in the cartilage of all the operated knees. Meniscal cells reverted to normal number, while active cell division decreased below normal, apoptotic events were still high, COMP remained elevated, and glycosaminoglycans were even more elevated.Extracellular matrix changes and altered cell distribution occur early in the degenerative meniscus. There is a close relationship between changes in the articular cartilage and the menisci at the onset of secondary OA.