Levels of Matrix-Degrading Enzymes and Lubricin in Patients With Degenerative Joint Disease Requiring Arthroplasty.
Levels of Matrix-Degrading Enzymes and Lubricin in Patients With Degenerative Joint Disease Requiring Arthroplasty.
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DOI：
10.1177/1076029617724231
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Hopkinson W
中科院分区：
文献类型：
--
作者：
Wanderling C;Liles J;Davis E;Schmitt D;Statz S;Guler N;Hoppensteadt D;Fareed J;Hopkinson W
髋关节或膝关节的全关节置换术 (TJA)（THA 和 TKA）是退行性关节病 (DJD) 患者的主要手术干预方法。尽管人们普遍认为关节上的剪切力会导致关节软骨退化，但可能还有其他因素导致 DJD 的进展。降解关节的特定酶可能上调，或者相反，对关节润滑至关重要的酶下调。本研究的目的是分析接受 TJA 的患者的胶原酶 1、弹性蛋白酶、乙酰肝素酶和润滑素水平，以确定潜在的预先存在的导致 DJD 发病机制的失调。在接受 TJA 前 1 天和术后 1 天从接受 TJA 的患者中获取已鉴定的血样。使用酶联免疫吸附测定试剂盒对血浆样品进行弹性蛋白酶、胶原酶-1、乙酰肝素酶和润滑素分析。与健康对照相比，术前和术后样本中循环胶原酶-1、弹性蛋白酶和润滑素水平均显着增加。术前或术后样本中乙酰肝素酶水平没有显着差异。比较术前与术后患者样本，只有润滑素表现出显着变化。这项研究的结果证实，接受 TJA 的患者基质降解酶和润滑素水平已存在改变。本研究中观察到的变化可能有助于深入了解 DJD 的发病机制。
Total joint arthroplasty (TJA) of the hip or knee (THA and TKA) is the primary surgical intervention for individuals with degenerative joint disease (DJD). Although it is commonly thought that shear force on the joint causes the degradation of articular cartilage, it is possible that there are other factors that contribute to the progression of DJD. It is plausible that specific enzymes that degrade the joint are upregulated, or conversely, there is downregulation of enzymes critical for joint lubrication. The aim of this study is to profile collagenase-1, elastase, heparanase, and lubricin levels in patients undergoing TJA in order to determine potential preexisting dysregulation that contributes to the pathogenesis of DJD. Deidentified blood samples were obtained from patients undergoing TJA 1 daypre-and 1 day postoperatively. Plasma samples were analyzed using enzyme-linked immunosorbent assay kits for elastase, collagenase-1, heparanase, and lubricin. In comparison to healthy controls, there were significant increases in circulating collagenase-1, elastase, and lubricin levels in both the preoperative and postoperative samples. There were no significant differences in heparanase levels in the preoperative or postoperative samples. Comparing the preoperative versus postoperative patient samples, only lubricin demonstrated a significant change. The results of this study confirm that patients undergoing TJA have preexisting alterations in the levels of matrix-degrading enzymes and lubricin. The alterations observed in this study may provide insight into the pathogenesis of DJD.
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影响因子：
3.7
作者：
Nakagawa Y;Muneta T;Otabe K;Ozeki N;Mizuno M;Udo M;Saito R;Yanagisawa K;Ichinose S;Koga H;Tsuji K;Sekiya I
通讯作者：
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影响因子：
--
作者：
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影响因子：
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作者：
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Dreier R
DOI：
10.1186/ar380
发表时间：
2002
期刊：
Arthritis research
影响因子：
--
作者：
Eyre D
通讯作者：
Eyre D
影响因子：
2.9
作者：
Burleson, Andrew;Guler, Nil;Hopkinson, William
通讯作者：
Hopkinson, William