X-ray crystal structure analysis of magnetically oriented microcrystals of lysozyme at 1.8 Å resolution
X-ray crystal structure analysis of magnetically oriented microcrystals of lysozyme at 1.8 Å resolution
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1.8 溶菌酶磁取向微晶的 X 射线晶体结构分析
DOI：
10.1107/s1600576716001357
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发表时间：
2016
影响因子：
6.1
通讯作者：
T
中科院分区：
文献类型：
--
作者：
Tsukui;S.;Kimura;F.;Garman;E.;Baba;S.;Mizuno;N.;Mikami;B.;& Kimura;T
将溶菌酶微晶（5-10 μm）悬浮在紫外光固化树脂中，在非均匀旋转磁场下三维排列，然后通过光聚合固结树脂，得到微晶三维排列的复合材料。使用同步加速器X射线源分析所获得的复合材料（MOMA：磁定向微晶阵列）。获得了 1.8 Å 的分辨率，这与之前报道的使用内部 X 射线衍射仪获得的这些复合材料的 3 Å 分辨率形成鲜明对比。这些结果表明，MOMA 与传统同步加速器光束线的组合可能对不生长到更大尺寸的蛋白质晶体的晶体分析具有宝贵的潜力。
Microcrystals of lysozyme (5–10 µm) suspended in an ultraviolet-light-curable resin were aligned three dimensionally under a non-uniformly rotating magnetic field, and then the resin was consolidated by photopolymerization to obtain a composite in which microcrystals were three-dimensionally aligned. The obtained composite (MOMA: magnetically oriented microcrystal array) was analysed using a synchrotron X-ray source. A resolution of 1.8 Å was obtained, which is in marked contrast to the resolution of 3 Å previously reported for these composites, obtained by using an in-house X-ray diffractometer. These results show that a combination of MOMAs with conventional synchrotron beamlines may have valuable potential for crystal analyses of protein crystals that do not grow to larger sizes.