Identification of protective and non-protective T cell epitopes in influenza
Identification of protective and non-protective T cell epitopes in influenza
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DOI：
10.1016/j.vaccine.2005.07.090
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发表时间：
2006-01-23
期刊：
影响因子：
5.5
通讯作者：
Woodland, DL
中科院分区：
文献类型：
--
作者：
Crowe, SR;Miller, SC;Woodland, DL
了解对不同CD 8 T细胞表位的免疫应答对于开发旨在促进保护性细胞免疫的疫苗非常重要。最近，我们已经表明，用流感病毒的PA（224-233）/D-b表位接种疫苗在病毒清除方面的保护性较差。为了确定其他流感病毒表位是否以这种方式表现，我们分析了三种新鉴定的CD 8 T细胞表位和三种先前定义的表位在疫苗接种和病毒攻击后提供保护的能力。所有六种基于肽的疫苗接种导致脾脏中表位特异性CD 8 T细胞的数量显著增加。有趣的是，我们发现用三种肽（HA（332-340）、M1（128-135）或PA（224-233））接种导致感染后病毒清除延迟。这些发现表明，某些表位对病毒清除有不利影响，并对旨在提供针对随后流感病毒攻击的保护的疫苗接种策略的发展具有重要意义。(c)2005爱思唯尔有限公司保留所有权利。
Understanding the immune response to different CD8 T cell epitopes is important for the development of vaccines designed to promote protective cellular immunity. Recently, we have shown that vaccination with the PA(224-233)/D-b epitope of influenza virus was poorly protective in terms of viral clearance. To determine if other influenza virus epitopes behave in this manner, we analyzed the ability of three newly identified CD8 T cell epitopes and three previously defined epitopes to provide protection following vaccination and viral challenge. All six of the peptide-based vaccinations resulted in significantly increased numbers of epitope-specific CD8 T cells in the spleen. Interestingly, we found that vaccination with three peptides (HA(332-340), M1(128-135), or PA(224-233)) resulted in delayed viral clearance following infection. These findings indicate that some epitopes have a detrimental impact on viral clearance and have important implications for the development of vaccination strategies designed to provide protection against subsequent influenza virus challenge. (c) 2005 Elsevier Ltd. All rights reserved.