High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources
High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources
复制标题
DOI：
10.3791/54908
复制
发表时间：
2016-11-01
影响因子：
1.2
通讯作者：
McKinstry-Wu, Andrew R.
中科院分区：
文献类型：
--
作者：
Wasilczuk, Andrzej Z.;Proekt, Alexander;McKinstry-Wu, Andrew R.
需要高空间分辨率的先进脑电图分析技术，包括电源成像和网络连接的措施，适用于神经科学中不断扩大的各种问题。在啮齿动物模型中执行这些分析需要比传统螺旋电极更高的电极密度。虽然存在用于啮齿动物的更高密度脑电图蒙太奇，但它们对大多数研究人员的可用性有限，对于长时间的重复实验不够稳健，或者仅限于在麻醉啮齿动物中使用。(1-3)提出了一种低成本的方法，用于构建一个持久的，高计数，经颅电极阵列，包括双边植入式头片进行先进的脑电图分析在小鼠或rates.Procedures头片制造和手术植入产生高信号噪声，低阻抗脑电图和肌电图信号。虽然该方法在大鼠和小鼠中都是有用的，但本文重点关注较小小鼠头骨的更具挑战性的实施。在录制过程中，自由移动的鼠标只能通过普通适配器连接到电缆上。下面描述包括26个脑电图通道和4个肌电图通道的该电极系统的一个版本。
Advanced electroencephalographic analysis techniques requiring high spatial resolution, including electrical source imaging and measures of network connectivity, are applicable to an expanding variety of questions in neuroscience. Performing these kinds of analyses in a rodent model requires higher electrode density than traditional screw electrodes can accomplish. While higher-density electroencephalographic montages for rodents exist, they are of limited availability to most researchers, are not robust enough for repeated experiments over an extended period of time, or are limited to use in anesthetized rodents. (1-3) A proposed low-cost method for constructing a durable, high-count, transcranial electrode array, consisting of bilaterally implantable headpieces is investigated as a means to perform advanced electroencephalogram analyses in mice or rats.Procedures for headpiece fabrication and surgical implantation necessary to produce high signal to noise, low-impedance electroencephalographic and electromyographic signals are presented. While the methodology is useful in both rats and mice, this manuscript focuses on the more challenging implementation for the smaller mouse skull. Freely moving mice are only tethered to cables via a common adapter during recording. One version of this electrode system that includes 26 electroencephalographic channels and 4 electromyographic channels is described below.