Bacterial detection based on polymerase chain reaction and microbead dielectrophoresis characteristics
Bacterial detection based on polymerase chain reaction and microbead dielectrophoresis characteristics
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DOI：
10.1049/iet-nbt.2016.0186
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发表时间：
2017-08-01
影响因子：
2.3
通讯作者：
Suehiro, Junya
中科院分区：
文献类型：
--
作者：
Ding, Zhenhao;Kasahara, Hiromichi;Suehiro, Junya
在这项研究中，一种电DNA检测方法被应用于细菌检测。从细菌中提取DNA，并通过聚合酶链式反应进行扩增。微珠用扩增子标记，改变了它们的表面电导，进而改变了它们的介电泳特性。因此，扩增子标记的微珠可以被捕获在高强度电场中，在电极之间形成珍珠链，导致电极之间的电导增加。与传统方法相比，这种方法将扩增子检测时间从1 - 2小时缩短到15分钟。所提出的方法分别在有和没有其他细菌存在的细菌溶液中，实现了对浓度高于1×10⁵和2.4×10⁴CFU/ml的特定细菌的定量检测。
In this study, an electrical DNA detection method was applied to bacterial detection. DNA was extracted from bacteria and amplified by polymerase chain reaction. The microbeads were labelled with amplicons, altering their surface conductance and therefore their dielectrophoresis characteristics. Amplicon-labelled microbeads could thus be trapped within a high-strength electric field, where they formed a pearl chain between the electrodes, resulting in an increased conductance between the electrodes. This method reduces the amplicon detection time from 1-2 h to 15 min, compared with the conventional method. The presented method realised quantitative detection of specific bacteria at concentrations above 1 x 10(5) and 2.4 x 10(4) CFU/ml for bacterial solutions with and without other bacterial presence, respectively.