Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages.
Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages.
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分析代表不同非生物应力，组织类型和发育阶段的高羊茅。
DOI：
10.1186/1471-2229-8-27
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发表时间：
2008-03-04
影响因子：
5.3
通讯作者：
May, Gregory D.
中科院分区：
文献类型：
--
作者：
Mian, M. A. Rouf;Zhang, Yan;Wang, Zeng-Yu;Zhang, Ji-Yi;Cheng, Xiaofei;Chen, Lei;Chekhovskiy, Konstantin;Dai, Xinbin;Mao, Chunhong;Cheung, Foo;Zhao, Xuechun;He, Ji;Scott, Angela D.;Town, Christopher D.;May, Gregory D.
高羊茅（Festuca arundinacea Schreb）是生长在世界温带地区的主要冷季饲料和草坪草品种。在本文中，我们报告了从代表不同植物器官、发育阶段和非生物胁迫因素的组织的九个 cDNA 文库开发的高羊茅表达序列标签 (EST) 数据库的生成。还报告了这些 EST 的文库间和文库特异性计算机表达分析的结果。从高羊茅的 9 个 cDNA 文库中生成了总共 41,516 个 EST，这些高羊茅代表来自不同植物器官、发育阶段和非生物胁迫条件的组织。羊茅基因索引（FaGI）已经建立。迄今为止，这是第一个公开可用的高羊茅 EST 数据库。使用这些 EST 进行计算机基因表达研究，以了解高羊茅的应激反应。从应激组织文库中鉴定出大量已知应激反应基因的EST。这些 EST 代表热休克和氧化应激蛋白以及各种转录因子蛋白家族的基因同源物。在应激组织文库中也鉴定出了代表未知功能基因的高表达 EST。 FaGI 为高羊茅和其他密切相关的饲料和草坪草物种的基因组学研究提供了有用的资源。高羊茅与其他草种（包括黑麦草 (Lolium sp.)、草甸羊茅 (F. pratensis) 和四倍体羊茅 (F. arundinacea var glaucescens)）之间的比较基因组分析将受益于该数据库。这些 EST 是开发基于简单序列重复 (SSR) 和单核苷酸多态性 (SNP) PCR 的分子标记的极好资源。
Tall fescue (Festuca arundinacea Schreb) is a major cool season forage and turf grass species grown in the temperate regions of the world. In this paper we report the generation of a tall fescue expressed sequence tag (EST) database developed from nine cDNA libraries representing tissues from different plant organs, developmental stages, and abiotic stress factors. The results of inter-library and library-specific in silico expression analyses of these ESTs are also reported. A total of 41,516 ESTs were generated from nine cDNA libraries of tall fescue representing tissues from different plant organs, developmental stages, and abiotic stress conditions. The Festuca Gene Index (FaGI) has been established. To date, this represents the first publicly available tall fescue EST database. In silico gene expression studies using these ESTs were performed to understand stress responses in tall fescue. A large number of ESTs of known stress response gene were identified from stressed tissue libraries. These ESTs represent gene homologues of heat-shock and oxidative stress proteins, and various transcription factor protein families. Highly expressed ESTs representing genes of unknown functions were also identified in the stressed tissue libraries. FaGI provides a useful resource for genomics studies of tall fescue and other closely related forage and turf grass species. Comparative genomic analyses between tall fescue and other grass species, including ryegrasses (Lolium sp.), meadow fescue (F. pratensis) and tetraploid fescue (F. arundinacea var glaucescens) will benefit from this database. These ESTs are an excellent resource for the development of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) PCR-based molecular markers.
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影响因子：
4.8
作者：
Almoguera, C;Rojas, A;Jordano, J
通讯作者：
Jordano, J
影响因子：
4.1
作者：
Morris, CA;Nicolaus, B;Kille, P
通讯作者：
Kille, P
影响因子：
5.4
作者：
Saha, MC;Mian, R;Hopkins, AA
通讯作者：
Hopkins, AA
影响因子：
7.4
作者：
Gaudin, V;Lunness, PA;Doonan, JH
通讯作者：
Doonan, JH
影响因子：
3.9
作者：
Karlin, S;Brocchieri, L
通讯作者：
Brocchieri, L