A simple microfluidic chip design for fundamental bioseparation.
A simple microfluidic chip design for fundamental bioseparation.
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DOI：
10.1155/2014/175457
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发表时间：
2014
影响因子：
2.6
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Chan AS;Danquah MK;Agyei D;Hartley PG;Zhu Y
以聚二甲基硅氧烷(PDMS)为填充柱，设计并制作了一种微芯片压力驱动液相色谱系统。色谱柱为Ia3d空间群介孔二氧化硅小球。为了验证芯片的性能，对染料和生物聚合物进行了分离。分离了染料(荧光素和罗丹明B)和生物聚合物混合物(10 葡聚糖和66 kDa牛血清白蛋白)，并用荧光技术检测了分子的运动。当染料混合物在去离子水中注入到微通道中时，荧光素分子是非滞留物种，罗丹明B附着在二氧化硅表面。在生物聚合物分离实验中，葡聚糖分子和牛血清白蛋白分子的保留时间分别为45 S和120 S。葡聚糖的保留因子为3.3，牛血清白蛋白的保留因子为10.4。选择性为3.2，分离度为10.7。实现了染料和生物聚合物的良好分离，验证了芯片的设计。
A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluorescein and rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified.