Subcellular localization of the alpha(7) nicotinic receptor in rat cerebellar granule cell layer
Subcellular localization of the alpha(7) nicotinic receptor in rat cerebellar granule cell layer
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DOI：
10.1097/00001756-199704140-00021
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发表时间：
1997-04-14
期刊：
影响因子：
1.7
通讯作者：
Pesold, C
中科院分区：
文献类型：
--
作者：
Caruncho, HJ;Guidotti, A;Pesold, C
用α(7)亚基特异性抗体在电镜下免疫组织化学方法研究了α(7)烟碱受体亚基在大鼠小脑中的分布。颗粒细胞层对α(7)亚基的免疫反应性比浦肯野细胞层低得多。颗粒细胞体完全无标记；这似乎仅限于位于颗粒细胞树突膜的肾小球。α(7)免疫标记不位于活跃的突触区，而主要位于突触周围。这种定位表明含有α(7)亚基的尼古丁受体可能具有调节功能和/或在突触电流的产生中起直接作用。
The distribution of the alpha(7) nicotinic receptor subunit in the rat cerebellum was studied immunohistochemically at the electron microscope level using an alpha(7) subunit-specific antibody. The granule cell layer showed a much lower level of immunoreactivity for the alpha(7) subunit than the Purkinje cell layer. Granule cell somata were completely devoid of labeling; this appeared to be restricted to glomeruli exclusively located in the membranes of granule cell dendrites. The alpha(7) immunolabeling was located not at active synaptic areas but was mostly perisynaptic. This localization suggests that nicotinic receptors containing the alpha(7) subunit could have a modulatory function and/or play a direct role in the generation of synaptic currents.