Gene silencing in human aortic smooth muscle cells induced by PEI-siRNA complexes released from dip-coated electrospun poly(ethylene terephthalate) grafts.
Gene silencing in human aortic smooth muscle cells induced by PEI-siRNA complexes released from dip-coated electrospun poly(ethylene terephthalate) grafts.
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DOI：
10.1016/j.biomaterials.2013.12.026
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发表时间：
2014-03
期刊：
影响因子：
14
通讯作者：
LoGerfo, Frank W.
中科院分区：
文献类型：
--
作者：
Nabzdyk, Christoph S.;Chun, Maggie C.;Oliver-Allen, Hunter S.;Pathan, Saif G.;Phaneuf, Matthew D.;You, Jin-Oh;Pradhan-Nabzdyk, Leena K.;LoGerfo, Frank W.
组织对人工动脉移植材料的过度反应会导致内膜增生（IH），这是晚期移植失败的主要原因。假体材料植入后也可能发生血清肿和异常包膜形成。基质细胞蛋白 Thrombospondin-2 (TSP-2) 已被证明会因生物材料植入而上调。本研究评估了未修饰和表面功能化电纺 PET 移植材料中小干扰 RNA (siRNA) 的吸收和释放。采用静电纺丝技术合成了 ePET 接枝材料。然后对 ePET 材料的子集进行化学改性以产生表面官能团。将未修饰和表面修饰的 ePET 移植物浸涂在单独的 siRNA 中或与转染试剂聚乙烯亚胺 (PEI) 或 Lipofectamine RNAiMax 复合的 siRNA 中。此外，将对照和 TSP-2 siRNA-PEI 复合物处理的 ePET 样品置于人主动脉平滑肌细胞 (AoSMC) 的汇合层上。所有 siRNA 与 PEI 的复合导致对未修饰 ePET 的吸附显着增加。从未修饰的 ePET 中释放的 TSP-2 siRNA-PEI 沉默了 AoSMC 中的 TSP-2。无论评估的 siRNA-PEI 复合物如何，AoSMC 都会迁移到 ePET 中。从浸涂的 ePET 递送至 AoSMC 的 siRNA-PEI 复合物可导致基因敲低。这种 siRNA 递送方法可以改善组织对血管和其他假体的反应。
An excessive tissue response to prosthetic arterial graft material leads to intimal hyperplasia (IH), the leading cause of late graft failure. Seroma and abnormal capsule formation may also occur after prosthetic material implantation. The matricellular protein Thrombospondin-2 (TSP-2) has shown to be upregulated in response to biomaterial implantation. This study evaluates the uptake and release of small interfering RNA (siRNA) from unmodified and surface functionalized electrospun PET graft materials. ePET graft materials were synthesized using electrospinning technology. Subsets of the ePET materials were then chemically modified to create surface functional groups. Unmodified and surface-modified ePET grafts were dip-coated in siRNAs alone or siRNAs complexed with transfection reagents polyethyleneimine (PEI) or Lipofectamine RNAiMax. Further, control and TSP-2 siRNA-PEI complex treated ePET samples were placed onto a confluent layer of human aortic smooth muscle cells (AoSMCs). Complexation of all siRNAs with PEI led to a significant increase in adsorption to unmodified ePET. TSP-2 siRNA-PEI released from unmodified-ePET silenced TSP-2 in AoSMC. Regardless of the siRNA-PEI complex evaluated, AoSMC migrated into the ePET. siRNA-PEI complexes delivered to AoSMC from dip-coated ePET can result in gene knock-down. This methodology for siRNA delivery may improve the tissue response to vascular and other prosthetics.
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影响因子：
4.8
作者：
Monahan, Thomas S.;Andersen, Nicholas D.;LoGerfo, Frank W.
通讯作者：
LoGerfo, Frank W.
影响因子：
2.6
作者：
Mehrabi, Mostafa;Jangjoo, Ali;Kahrom, Hadi
通讯作者：
Kahrom, Hadi
DOI：
10.1016/j.jamcollsurg.2006.12.029
发表时间：
2007-03-01
影响因子：
5.2
作者：
Andersen, Nicholas D.;Monahan, Thomas S.;LoGerfo, Frank W.
通讯作者：
LoGerfo, Frank W.
影响因子：
2.1
作者：
Bouillot, J. -L.;Pogoshian, T.;Veyrie, N.
通讯作者：
Veyrie, N.
影响因子：
5.7
作者：
Nolte, Andrea;Walker, Tobias;Wendel, Hans Peter
通讯作者：
Wendel, Hans Peter